Increase in short telomeres during the third trimester in human placenta.
Increase in short telomeres during the third trimester in human placenta.
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DOI：
10.1371/journal.pone.0271415
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Phillippe, Mark
中科院分区：
文献类型：
--
作者：
Edelson, Paula K.;Sawyer, Michala R.;Gray, Kathryn J.;Cantonwine, David E.;McElrath, Thomas F.;Phillippe, Mark
妊娠组织中端粒缩短的增加被认为是涉及分娩开始时间的机制。在小鼠中观察到，随着胎龄的增加，非常短的端粒也随之增加。这项研究试图探讨人类怀孕中的这一现象。具体来说，这项研究提出了这样的假设：在临产前，随着足月妊娠的临近，人类胎盘组织中非常短的端粒（长度<3 kb）的数量会增加。主要结果是胎盘组织中存在的非常短端粒的数量。使用端粒限制性片段技术的实时聚合酶链反应（qPCR）对非常短的端粒进行定量测量。包括 69 名孕妇的胎盘组织。平均孕龄为 39.1 周（足月）和 36.2 周（早产）。对于足月胎盘与早产胎盘，观察到的极短端粒增加如下：500 bp 端粒增加 1.67 倍（p < 0.03）； 1 kb 端粒增加 1.67 倍 (p < 0.08)； 3 kb 端粒增加了 5.20 倍 (p < 0.001)。这项研究证实足月时人类胎盘组织中非常短的端粒显着增加；从而支持了这样的假设：足月端粒缩短有助于决定妊娠长度的机制，从而导致分娩开始。
An increase in telomere shortening in gestational tissues has been proposed as a mechanism involved in the timing for the initiation of parturition. An increase in very short telomeres with increasing gestational age has been observed in mice; this study sought to explore this phenomenon in human pregnancies. Specifically, this study addressed the hypothesis that prior to labor, the quantity of very short telomeres (<3 kilobase (kb) lengths) increases in human placental tissue as term gestation approaches. The primary outcome was the quantity of very short telomeres present in placental tissue. Quantitative measurements of very short telomeres were performed using real-time polymerase chain reaction (qPCR) adaptation of the telomere restriction fragment technique. Placental tissue from 69 pregnant individuals were included. Mean gestational age was 39.1 weeks (term) and 36.2 weeks (preterm). For term versus preterm placentas, the observed increase in very short telomeres were as follows: 500 bp telomeres increased by 1.67-fold (p < 0.03); 1 kb telomeres increased 1.67-fold (p < 0.08); and 3 kb telomeres increased 5.20-fold (p < 0.001). This study confirms a significant increase in very short telomeres in human placental tissue at term; thereby supporting the hypothesis that telomere shortening at term contributes to the mechanism that determine the length of pregnancy thereby leading to onset of parturition.
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