Functional Replacement of the Intracellular Region of the Notch1 Receptor by Epstein-Barr Virus Nuclear Antigen 2
Functional Replacement of the Intracellular Region of the Notch1 Receptor by Epstein-Barr Virus Nuclear Antigen 2
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Epstein-Barr 病毒核抗原 2 对 Notch1 受体细胞内区域的功能替换
DOI：
10.1128/jvi.72.7.6034-6039.1998
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发表时间：
1998
影响因子：
5.4
通讯作者：
T. Honjo
中科院分区：
文献类型：
--
作者：
T. Sakai;Yoshihito Taniguchi;K. Tamura;S. Minoguchi;T. Fukuhara;L. Strobl;U. Zimber;G. Bornkamm;T. Honjo
摘要小鼠Notch1受体的胞内区(Ramic)与RBP-J/CBF-1相互作用，RBP-J/CBF-1与DNA序列CGTGGGAA结合，通过转录激活受RBP-J调控的基因而抑制分化。EB病毒核抗原2(EBNA2)是病毒使人B细胞永生化所必需的。EBNA2是病毒和细胞基因的多效性激活剂，至少部分通过与RBP-J相互作用而靶向DNA。我们发现EBNA2和Notch1 Ramic竞争与RBP-J结合，表明它们在RBP-J上的相互作用部位至少部分重叠。EBNA2和Notch1 Ramic反式激活同一组病毒和宿主启动子，即Epstein-Barr病毒TP1的EBNA2反应元件和HES-1启动子。此外，EBNA2通过抑制C2C12成肌祖细胞的分化，在功能上取代了Notch1 Ramic。
ABSTRACT The intracellular region (RAMIC) of the mouse Notch1 receptor interacts with RBP-J/CBF-1, which binds to the DNA sequence CGTGGGAA and suppresses differentiation by transcriptional activation of genes regulated by RBP-J. Epstein-Barr virus nuclear antigen 2 (EBNA2) is essential for immortalization of human B cells by the virus. EBNA2 is a pleiotropic activator of viral and cellular genes and is targeted to DNA at least in part by interacting with RBP-J. We found that EBNA2 and the Notch1 RAMIC compete for binding to RBP-J, indicating that their interaction sites on RBP-J overlap at least partially. EBNA2 and Notch1 RAMIC transactivated the same set of viral and host promoters, i.e., the EBNA2 response element of the Epstein-Barr virus TP1 and the HES-1 promoter. Furthermore, EBNA2 functionally replaced the Notch1 RAMIC by suppressing differentiation of C2C12 myoblast progenitor cells.
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遗传和生化证据表明，EBNA 2 与 63 kDa 细胞 GTG 结合蛋白的相互作用对于 EBV 引起的 B 淋巴细胞生长转化至关重要。
影响因子：
3.7
作者：
Yalamanchili,R;Tong,X;Grossman,S;Johannsen,E;Mosialos,G;Kieff,E
通讯作者：
Kieff,E
DOI：
10.1073/pnas.91.16.7568
发表时间：
1994-08-02
影响因子：
11.1
作者：
GROSSMAN, SR;JOHANNSEN, E;KIEFF, E
通讯作者：
KIEFF, E
DOI：
10.1073/pnas.90.20.9237
发表时间：
1993-10-15
影响因子：
11.1
作者：
LING, PD;RAWLINS, DR;HAYWARD, SD
通讯作者：
HAYWARD, SD
影响因子：
56.9
作者：
HENKEL, T;LING, PD;PETERSON, MG
通讯作者：
PETERSON, MG