Placental lipases in pregnancies complicated by gestational diabetes mellitus (GDM).
Placental lipases in pregnancies complicated by gestational diabetes mellitus (GDM).
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DOI：
10.1371/journal.pone.0104826
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Nitert MD
中科院分区：
文献类型：
--
作者：
Barrett HL;Kubala MH;Scholz Romero K;Denny KJ;Woodruff TM;McIntyre HD;Callaway LK;Nitert MD
患有妊娠糖尿病（GDM）的妇女所生的婴儿更有可能比胎龄大，体脂百分比更高。母体脂质升高可能对此有贡献。胎盘脂肪酶如脂蛋白脂肪酶（LPL）、内皮脂肪酶（EL）和激素敏感脂肪酶（HSL）参与将脂质从母体转移到胎儿。以前的研究表明，这些脂肪酶在妊娠期糖尿病妇女胎盘中的表达有不同的结果。细胞内脂肪酶如脂肪甘油三酯脂肪酶（ATGL）和HSL是脂滴代谢的中心。这些脂肪酶的活性都受到Perilipin 1的影响，ATGL也被辅因子比较基因识别-58（CGI-58）激活，并被G 0/G1转换基因2（GS 02）抑制。这些修饰因子或ATGL以前在胎盘中均未检测到。因此，本研究的目的是检查ATGL，HSL，LPL，EL，以及Perilipin 1，GS 02和CGI-58在足月妊娠并发GDM中的表达。mRNA和蛋白质表达的脂肪酶测定胎盘从17名妇女与GDM和17名正常妊娠，母亲的BMI和分娩的胎龄相匹配。ATGL mRNA的表达增加，HSL mRNA的表达减少胎盘从GDM，虽然没有任何脂肪酶的蛋白表达的差异。所有脂肪酶定位于滋养层细胞和内皮细胞。GDM组Perilipin 1和CGI-58 mRNA表达增加，GS 02表达无明显变化。这些结果表明，在GDM和正常血糖胎盘之间，这四种脂肪酶的表达没有差异，因此通过这些脂肪酶改变的脂质转移不会导致GDM妇女的婴儿大于胎龄。
Infants of women with gestational diabetes mellitus (GDM) are more likely to be born large for gestational age with a higher percentage body fat. Elevated maternal lipids may contribute to this. Placental lipases such as lipoprotein lipase (LPL), endothelial lipase (EL) and hormone sensitive lipase (HSL) are involved in transferring lipids from mother to fetus. Previous studies of expression of these lipases in placentae in women with diabetes in pregnancy have reported divergent results. Intracellular lipases such as adipose triglyceride lipase (ATGL), and HSL are central to lipid droplet metabolism. The activities of these lipases are both influenced by Perilipin 1, and ATGL is also activated by a co-factor comparative gene identification-58 (CGI-58) and inhibited by G0/G1 switch gene 2 (GS02). None of these modifying factors or ATGL have been examined previously in placenta. The purpose of this study was therefore to examine the expression of ATGL, HSL, LPL, EL, as well as Perilipin 1, GS02 and CGI-58 in term pregnancies complicated by GDM. mRNA and protein expression of the lipases were measured in placentae from 17 women with GDM and 17 normoglycaemic pregnancies, matched for maternal BMI and gestational age of delivery. ATGL mRNA expression was increased and HSL mRNA expression reduced in placentae from GDM although there was no differences in protein expression of any of the lipases. All lipases were localised to trophoblasts and endothelial cells. The expression of Perilipin 1 and CGI-58 mRNA was increased and GS02 not altered in GDM. These results suggest that there is no difference in expression in these four lipases between GDM and normoglycaemic placentae, and therefore altered lipid transfer via these lipases does not contribute to large for gestational age in infants of women with GDM.
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