Human amniotic membrane-derived mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles: the effect on neuron-like differentiation in vitro
Human amniotic membrane-derived mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles: the effect on neuron-like differentiation in vitro
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超顺磁性氧化铁纳米粒子标记的人羊膜间充质干细胞：对体外神经元样分化的影响
DOI：
10.1007/s11010-011-0904-4
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发表时间：
2011-05
影响因子：
4.3
通讯作者：
Bo Yang
中科院分区：
文献类型：
--
作者：
Guangwei Zeng;Gongping Wang;Fangxia Guan;Keliang Chang;Hongliang Jiao;Wanqin Gao;Shoumin Xi;Bo Yang
间充质干细胞(MSCs)具有自我更新和多向分化潜能，可再生受损组织或修复疾病中的功能缺失。超顺磁性氧化铁纳米颗粒(SPION)被用作磁共振成像(MRI)中的造影剂，用于体外标记细胞和体内移植后示踪Spion标记的细胞。人羊膜间充质干细胞(HAM-dMSCs)具有神经元样分化能力，可用于治疗中枢神经系统(CNS)疾病。本研究采用单次(1倍)和多次(4倍)SPION标记的方法，研究了SPION的细胞毒性对HAM-dMSCs神经元样分化的影响。在安全浓度的SPION(≤14和μg/ml)下，HAM-dMSCs可以被有效地标记，而不会显著影响其活性(经MTT80%检测)、特殊表面抗原(通过流式细胞术检测CD29、CD44、CD90、CD105)和神经元样分化(通过免疫细胞化学和逆转录聚合酶链式反应检测巢蛋白和神经元特异性烯醇化酶)。与多个(4×)Spion标记方法相比，单一(1×)Spion标记方法避免了多个Spion标记的Ham-dMSCs，并最大限度地减少了SPIOns对Ham-dMSCs神经元样分化的影响。在安全浓度下，单次(1×)SPIONS标记方法可为SPIONS标记的Ham-dMSCs提供合适的存活率，并有助于移植后Ham-dMSCs的MRI评估。
Mesenchymal stem cells (MSCs) have the potential for self-renewal and multipotential differentiation to regenerate damaged tissues or recover functional absence in diseases. Superparamagnetic iron oxide nanoparticles (SPIONs) are used as contrast agents in magnetic resonance imaging (MRI) for labeling cells in vitro and for tracking SPION-labeled cells after transplantation in vivo. Human amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) have the capacity for neuron-like differentiation that could be used to cure central nervous system (CNS) diseases. The study investigated the impacts of cytotoxicity of SPIONs on neuron-like differentiation of hAM-dMSCs in both single (1×) and multiple (4×) SPIONs-labeled methods. hAM-dMSCs could be efficiently labeled at safe concentrations of SPIONs (≤14 μg/ml) without significantly affecting their viability (>80% after a MTT assay), special surface antigens (CD29, CD44, CD90, CD105 through flow cytometry), and neuron-like differentiation (nestin and neuron-specific enolase through immunocytochemistry and reverse transcription polymerase chain reaction). Compared with multiple (4×) SPION-labeled methods, a single (1×) SPION-labeled method avoided multiple SPION-labeled hAM-dMSCs and minimized the impact of cytotoxicity of SPIONs on neuron-like differentiation of hAM-dMSCs. Under safe concentrations of SPIONs, a single (1×) SPION-labeled method provided appropriate viability for SPIONs-labeled hAM-dMSCs and facilitated the MRI evaluation of hAM-dMSCs after transplantation.
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DOI：
10.1016/j.lab.2005.07.003
发表时间：
2005-11-01
期刊：
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
影响因子：
--
作者：
Lu, FZ;Fujino, M;Li, XK
通讯作者：
Li, XK
影响因子：
5.2
作者：
Pawelczyk, Edyta;Arbab, Ali S.;Frank, Joseph A.
通讯作者：
Frank, Joseph A.
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
6.2
作者：
Zhao, P;Ise, H;Nikaido, T
通讯作者：
Nikaido, T
DOI：
--
发表时间：
2006
期刊：
Journal of Zhengzhou University
影响因子：
--
作者：
Li Guoxi
通讯作者：
Li Guoxi