An ion-exchange nanomembrane sensor for detection of nucleic acids using a surface charge inversion phenomenon.
An ion-exchange nanomembrane sensor for detection of nucleic acids using a surface charge inversion phenomenon.
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DOI：
10.1016/j.bios.2014.04.008
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发表时间：
2014-10-15
影响因子：
12.6
通讯作者：
Chang, Hsueh-Chia
中科院分区：
文献类型：
--
作者：
Senapati, Satyajyoti;Slouka, Zdenek;Shah, Sunny S.;Behura, Susanta K.;Shi, Zonggao;Stack, M. Sharon;Severson, David W.;Chang, Hsueh-Chia
We present a novel low-cost biosensor for rapid, sensitive and selective detection of nucleic acids based on an ionic diode feature of an anion exchange nanoporous membrane under DC bias. The ionic diode feature is associated with external surface charge inversion on the positively charged anion exchange nanomembrane upon hybridization of negatively charged nucleic acid molecules to single-stranded oligoprobes functionalized on the membrane surface resulting in the formation of a cation selective monolayer. The resulting bipolar membrane causes a transition from electroconvection-controlled to water-splitting controlled ion conductance, with a large ion current signature that can be used to accurately quantify the hybridized nucleic acids. The platform is capable of distinguishing two base-pair mismatches in a 22-base pairing segment of microRNAs associated with oral cancer, as well as serotype-specific detection of dengue virus. We also show the sensor’s capability to selectively capture target nucleic acids from a heterogeneous mixture. The limit of detection is 1 pM for short 27 base target molecules in a 15-minute assay. Similar hybridization results are shown for short DNA molecules as well as RNAs from Brucella and E.coli. The versatility and simplicity of this low-cost biosensor should enable point-of-care diagnostics in food, medical and environmental safety markets.
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DOI：
10.3390/s90403122
发表时间：
2009
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Cagnin S;Caraballo M;Guiducci C;Martini P;Ross M;Santaana M;Danley D;West T;Lanfranchi G
通讯作者：
Lanfranchi G
影响因子：
3.3
作者：
Belova, Elena I.;Lopatkova, Galina Yu.;Pourcelly, Gerald
通讯作者：
Pourcelly, Gerald
影响因子：
2.9
作者：
Lucas, SW;Harding, MM
通讯作者：
Harding, MM
影响因子：
3.2
作者：
Cheng, Li-Jing;Chang, Hsueh-Chia
通讯作者：
Chang, Hsueh-Chia
影响因子：
3.1
作者：
O'Connor, Louise;Glynn, Barry
通讯作者：
Glynn, Barry