Phasing of single DNA molecules by massively parallel barcoding.
Phasing of single DNA molecules by massively parallel barcoding.
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DOI：
10.1038/ncomms8173
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发表时间：
2015-06-09
影响因子：
16.6
通讯作者：
Ahmadian, Afshin
中科院分区：
文献类型：
--
作者：
Borgstroem, Erik;Redin, David;Lundin, Sverker;Berglund, Emelie;Andersson, Anders F.;Ahmadian, Afshin
高通量测序平台主要产生短读数据，导致许多分析的遗传变异的损失，对于某些应用，至关重要的是，知道哪些变体等位基因与每个单独的DNA分子相连单个DNA分子的大规模平行条形码和相位的方法。来自多个位置的生物学信息的耦合。复杂样品中的长单分子而不会丢失相信息。
DNA相相信息 - 哪些特定序列属于相同的DNA分子的确定不容易从依赖短读数的测序应用中获得。
High-throughput sequencing platforms mainly produce short-read data, resulting in a loss of phasing information for many of the genetic variants analysed. For certain applications, it is vital to know which variant alleles are connected to each individual DNA molecule. Here we demonstrate a method for massively parallel barcoding and phasing of single DNA molecules. First, a primer library with millions of uniquely barcoded beads is generated. When compartmentalized with single DNA molecules, the beads can be used to amplify and tag any target sequences of interest, enabling coupling of the biological information from multiple loci. We apply the assay to bacterial 16S sequencing and up to 94% of the hypothesized phasing events are shown to originate from single molecules. The method enables use of widely available short-read-sequencing platforms to study long single molecules within a complex sample, without losing phase information. DNA phasing information — the determination of which specific sequences belong to the same DNA molecule—is not easily obtained from sequencing applications that rely on short reads. Here the authors develop a phasing method based on massively parallel barcoding of single DNA molecules.
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影响因子：
7.7
作者：
Voskoboynik A;Neff NF;Sahoo D;Newman AM;Pushkarev D;Koh W;Passarelli B;Fan HC;Mantalas GL;Palmeri KJ;Ishizuka KJ;Gissi C;Griggio F;Ben-Shlomo R;Corey DM;Penland L;White RA 3rd;Weissman IL;Quake SR
通讯作者：
Quake SR
影响因子：
64.8
作者：
Peters, Brock A.;Kermani, Bahram G.;Sparks, Andrew B.;Alferov, Oleg;Hong, Peter;Alexeev, Andrei;Jiang, Yuan;Dahl, Fredrik;Tang, Y. Tom;Haas, Juergen;Robasky, Kimberly;Zaranek, Alexander Wait;Lee, Je-Hyuk;Ball, Madeleine Price;Peterson, Joseph E.;Perazich, Helena;Yeung, George;Liu, Jia;Chen, Linsu;Kennemer, Michael I.;Pothuraju, Kaliprasad;Konvicka, Karel;Tsoupko-Sitnikov, Mike;Pant, Krishna P.;Ebert, Jessica C.;Nilsen, Geoffrey B.;Baccash, Jonathan;Halpern, Aaron L.;Church, George M.;Drmanac, Radoje
通讯作者：
Drmanac, Radoje
影响因子：
48
作者：
Hiatt, Joseph B.;Patwardhan, Rupali P.;Turner, Emily H.;Lee, Choli;Shendure, Jay
通讯作者：
Shendure, Jay
DOI：
10.1101/sqb.2003.68.69
发表时间：
2003-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
Fan, JB;Oliphant, A;Chee, MS
通讯作者：
Chee, MS
影响因子：
3.7
作者：
Lundin, Sverker;Stranneheim, Henrik;Lundeberg, Joakim
通讯作者：
Lundeberg, Joakim