Oral administration of live- or heat-killed Candida albicans worsened cecal ligation and puncture sepsis in a murine model possibly due to an increased serum (1→3)-β-D-glucan.
Oral administration of live- or heat-killed Candida albicans worsened cecal ligation and puncture sepsis in a murine model possibly due to an increased serum (1→3)-β-D-glucan.
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DOI：
10.1371/journal.pone.0181439
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Leelahavanichkul A
中科院分区：
文献类型：
--
作者：
Panpetch W;Somboonna N;Bulan DE;Issara-Amphorn J;Finkelman M;Worasilchai N;Chindamporn A;Palaga T;Tumwasorn S;Leelahavanichkul A
白色念珠菌是人类肠道微生物群中最常见的真菌，但在小鼠中则不然。为了使小鼠脓毒症模型更接近人类脓毒症并探索肠道白色念珠菌在没有念珠菌血症的情况下在细菌性脓毒症中的作用，在盲肠结扎和穿刺（CLP）前3小时给小鼠口服活的或热灭活的白色念珠菌。在 CLP 之前给予念珠菌（活灭活或热灭活）证明 CLP 死亡率较高。粪便念珠菌仅在活念珠菌给药的实验中出现。尽管不存在念珠菌血症，但在 CLP 后 6 小时和/或 18 小时，给予念珠菌的 CLP 中的血清 (1→3)-β-D-葡聚糖 (BG) 高于 CLP 对照（给予生理盐水）。有趣的是，氟康唑减轻了粪便念珠菌负担，并提高了活念珠菌给药小鼠的存活率，但 CLP 对照小鼠的存活率没有改善。微生物群分析显示拟杆菌属增加。和减少的乳杆菌属。念珠菌给药后粪便中。此外，通过同时暴露于热灭活的大肠杆菌和 BG，在体外诱导骨髓源性巨噬细胞产生细胞因子方面具有协同作用。总之，肠道中真菌和/或真菌分子的丰度与细菌败血症严重程度的增加有关，这可能是通过对来自肠道的细菌和真菌分子的协同反应诱导的细胞因子诱导增强而实现的。相反，在我们的模型中，减少肠道真菌负荷会降低血清血糖并减轻脓毒症。
Candida albicans is the most common fungus in the human intestinal microbiota but not in mice. To make a murine sepsis model more closely resemble human sepsis and to explore the role of intestinal C. albicans, in the absence of candidemia, in bacterial sepsis, live- or heat-killed C. albicans was orally administered to mice at 3h prior to cecal ligation and puncture (CLP). A higher mortality rate of CLP was demonstrated with Candida-administration (live- or heat-killed) prior to CLP. Fecal Candida presented only in experiments with live-Candida administration. Despite the absence of candidemia, serum (1→3)-β-D-glucan (BG) was higher in CLP with Candida-administration than CLP-controls (normal saline administration) at 6h and/or 18h post-CLP. Interestingly, fluconazole attenuated the fecal Candida burden and improved survival in mice with live-Candida administration, but not CLP-control. Microbiota analysis revealed increased Bacteroides spp. and reduced Lactobacillus spp. in feces after Candida administration. Additionally, synergy in the elicitation of cytokine production from bone marrow-derived macrophages, in vitro, was demonstrated by co-exposure to heat-killed E. coli and BG. In conclusion, intestinal abundance of fungi and/or fungal-molecules was associated with increased bacterial sepsis-severity, perhaps through enhanced cytokine elicitation induced by synergistic responses to molecules from gut-derived bacteria and fungi. Conversely, reducing intestinal fungal burdens decreased serum BG and attenuated sepsis in our model.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
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DOI：
10.1016/j.surge.2012.03.003
发表时间：
2012-12
影响因子：
2.5
作者：
Deitch, Edwin A.
通讯作者：
Deitch, Edwin A.
影响因子：
3.6
作者：
Boonyatecha N;Sangphech N;Wongchana W;Kueanjinda P;Palaga T
通讯作者：
Palaga T
DOI：
10.1038/nri3552
发表时间：
2013-12
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Li XQ;Zhu YH;Zhang HF;Yue Y;Cai ZX;Lu QP;Zhang L;Weng XG;Zhang FJ;Zhou D;Yang JC;Wang JF
通讯作者：
Wang JF