Isoform diversity and tandem duplication of the glycoprotein A gene in ferret Pneumocystis carinii.
Isoform diversity and tandem duplication of the glycoprotein A gene in ferret Pneumocystis carinii.
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雪貂卡氏肺囊虫糖蛋白 A 基因的亚型多样性和串联重复。
DOI：
10.1093/dnares/2.2.77
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Haidaris,PJ
中科院分区：
文献类型：
--
作者：
Wright,TW;Bissoondial,TY;Haidaris,CG;Gigliotti,F;Haidaris,PJ
Two ferretP. cariniigpA cDNA clones were identified that reacted identically with a panel of anti-gpA monoclonal antibodies, although their nucleotide sequences were 22% divergent. Each clone hybridized to a single mRNA species of 3,600 nucleotides only inP. carinii-infected lung mRNA, but RT-PCR analysis demonstrated that these cDNA clones were derived from two distinct gpA mRNA transcripts. Further PCR analysis demonstrated that the ferretP. cariniigenome contains at least two gpA genes lying in tandem on a single chromosome separated by a 329-bp intergenic region. Based on the terminal gene sequences of this tandem repeat and the cDNA clones, a composite full-length ferretP. cariniigpA coding sequence was constructed. The intergenic region immediately downstream of the stop codon of the first gpA gene contains three putative polyadenylation signals, and constitutes the 3′ untranslated region (UTR) of the gpA mRNA. Primer extension of the gpA mRNA resulted in products extending 74 and 244 nucleotides into the 5′ UTR. However, the intergenic region lying greater than 25 nucleotides upstream of the first methionine of the second gpA gene was found to be absent from the 5′ UTR.
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影响因子：
6.4
作者：
WADA, M;SUNKIN, SM;NAKAMURA, Y
通讯作者：
NAKAMURA, Y
影响因子：
15.3
作者：
Harmsen, A G;Stankiewicz, M
通讯作者：
Stankiewicz, M
通过免疫印迹比较大鼠和人卡氏肺孢子虫的抗原特征。
影响因子：
4.4
作者：
P. Walzer;M. Linke
通讯作者：
M. Linke
使用蛋白质免疫印迹法分析人和大鼠抗体识别的大鼠卡氏肺孢子虫抗原
影响因子：
3.1
作者：
D. Graves;S. McNabb;M. Worley;T. Downs;M. Ivey
通讯作者：
M. Ivey
卡氏肺孢子虫共感染实验室大鼠的两种遗传变异的证据
影响因子：
9.4
作者：
M. Cushion;Jianxiong Zhang;Michael Kaselis;David Giuntoli;S. Stringer;James R. Stringer
通讯作者：
James R. Stringer