A rapid and inexpensive method for the isolation and identification of Giardia
A rapid and inexpensive method for the isolation and identification of Giardia
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一种快速且廉价的贾第鞭毛虫分离和鉴定方法
DOI：
10.1016/j.mex.2020.100998
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发表时间：
2020-07
期刊：
影响因子：
1.9
通讯作者：
Jianfan Wen
中科院分区：
文献类型：
--
作者：
Zhangxia Lyu;Jianfan Wen
贾第鞭毛虫是一种寄生于脊椎动物体内的肠道原虫，对人类和世界范围内的家畜都有严重危害，其流行病学已引起科学家们的关注。贾第鞭毛虫是一种以腹盘为主要媒介的寄生于宿主肠道表面的寄生虫，其寄生于宿主肠道表面的寄生虫，其寄生于宿主肠道表面的寄生虫是贾第鞭毛虫的主要寄生虫之一。并且它们在体外培养期间也粘附于培养管壁。根据这一现象，我们建立了一种分离贾第鞭毛虫的方法，并根据贾第鞭毛虫的特殊密度，建立了一种分离贾第鞭毛虫囊的方法。通过从宿主动物中分离鉴定贾第鞭毛虫，验证了该方法的有效性。为进一步开展该虫的流行病学研究及其他需要较纯活体作为材料的研究提供了参考。一种保持分离贾第鞭毛虫生理活性的高效分离方法。避免了其他生物的影响，具有高精度的鉴定方法。·低成本、快速和方便的方法。
This work details a protocol for the isolation ofGiardiaspecies from their host animals and their identification based on their morphological and molecular characteristics.Giardiaare intestinal protozoan parasites found in almost all vertebrates and the epidemiology ofGiardiahas attracted the attention of scientists due to their harm to humans and live stocks worldwide.Giardiatrophozoites adhere to the surface of host's intestines using their ventral disc, and they also adhere the culture tube wall during in vitro culturing. We developed a method of isolatingGiardiatrophozoites according to this phenomenon, and a method of isolatingGiardiacysts according to their special density as well. We validated the protocol by isolating and identifyingGiardiaspecies from their host animals, and all the results support that this methodology has a certain validity. It could help the further epidemiological researches and other researches requiring relatively pure living organism as materials of this harmful parasite.•An isolation method with high efficiency which remains the physiological activity of isolatedGiardia.•An identification method with high accuracy which avoids the influences from other organisms.•Low-cost, fast and convenient methodology.
仓鼠中的贾第鞭毛虫新种，1882 年（肉鞭毛目：Hexamitidae）
影响因子：
3.2
作者：
Lyu Z;Shao J;Xue M;Ye Q;Chen B;Qin Y;Wen J
通讯作者：
Wen J
影响因子：
3.2
作者：
Kiani-Salmi, Narges;Fattahi-Bafghi, Ali;Akrami-Mohajeri, Fateme
通讯作者：
Akrami-Mohajeri, Fateme
我国14个地区无尾两栖动物敏捷贾第鞭毛虫流行情况调查
DOI：
10.1016/j.ijppaw.2020.04.009
发表时间：
2020-08-01
影响因子：
1.8
作者：
Lyu, Zhangxia;Cheng, Jiaoni;Wen, Jianfan
通讯作者：
Wen, Jianfan