Dynamic patterns of circular and linear RNAs in maize hybrid and parental lines
Dynamic patterns of circular and linear RNAs in maize hybrid and parental lines
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玉米杂交种和亲本系中环状和线性 RNA 的动态模式
DOI：
10.1007/s00122-019-03489-9
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发表时间：
2019-11
影响因子：
5.4
通讯作者：
Li Lin
中科院分区：
文献类型：
--
作者：
Luo Zi;Qian Jia;Chen Sijia;Li Lin
杂种优势，又称杂种优势，已广泛应用于玉米等作物的农艺生产中。然而，杂种优势的分子机制尚不完全清楚。为了更全面地了解与杂种优势相关的转录组动力学，我们收集了两种广泛使用的玉米自交系及其f1杂交种V4生育期幼苗叶片的线性mRNA转录物和环状rna （circRNAs）的全面序列数据。我们检测到超过25000个表达基因，其中1200多个环状rna在三种基因型中表现出显著的表达水平差异。尽管大多数线性和环状转录本在杂交中表现出加性表达，但环状rna的表达更可能是非加性的。有趣的是，来自同一位点的线性转录本及其相应环状rna的水平在所有三种基因型中显示出显著的关系和协调的表达模式。值得注意的是，在杂交中，线性转录本的等位基因特异性表达与来自同一位点的环状rna的表达显著相关，这表明线性转录本和环状转录本之间存在潜在的调控串扰。我们的研究提供了对玉米经典杂交三胞胎中线性和环状转录组动态变化的更深入理解。
Hybrid vigor, also known as heterosis, has been widely utilized in agronomic production of maize (Zea maysL.) and other crops. However, the molecular mechanisms underlying heterosis are still not fully understood. To provide a more complete understanding of the transcriptomic dynamics associated with heterosis, we collected a comprehensive set of sequence data on linear mRNA transcripts and circular RNAs (circRNAs) from seedling leaves of two widely used maize inbred lines and their F1hybrid at the V4 growth stage. We detected over 25,000 expressed genes with more than 1200 circRNAs that showed dramatic and distinct variations in expression level across the three genotypes. Although most linear and circular transcripts exhibited additive expression in the hybrid, the expression of circRNAs was more likely to be nonadditive. Interestingly, the levels of linear transcripts and their corresponding circRNAs from the same loci showed a significant relationship and coordinated expression mode across all three genotypes. Notably, in the hybrid, allele-specific expression of linear transcripts was significantly associated with the expression of circRNAs from the same locus, suggesting potential regulatory cross talk between linear and circular transcripts. Our study provides a deeper understanding of dynamic variations for both the linear and circular transcriptome in a classical hybrid triplet of maize.
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