Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma.
Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma.
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DOI：
10.1371/journal.pone.0180620
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Shu CW
中科院分区：
文献类型：
--
作者：
Liu PF;Hu YC;Kang BH;Tseng YK;Wu PC;Liang CC;Hou YY;Fu TY;Liou HH;Hsieh IC;Ger LP;Shu CW
细胞凋亡在癌症的发展和恶性肿瘤中起着双重作用。凋亡相关的半胱天冬酶在癌症中的作用仍然存在争议，特别是在口腔舌鳞状细胞癌（OTSCC）中。在这项研究中，我们通过免疫组织化学方法检测了246例OTSCC患者组织微阵列上切割的caspase-3、caspase-3、caspase-8和caspase-9的蛋白水平。Wilcoxon符号秩检验表明，与邻近正常组织相比，肿瘤组织中切割的caspase-3、caspase-3、caspase-8和caspase-9的蛋白水平显著较高（均p<0.001）。caspase-8在有淋巴结浸润的患者中表达水平升高。此外，在具有中等分化和淋巴结浸润的OTSCC患者中，裂解型caspase-3的阳性表达与较短的无病生存期（DFS）相关。裂解型caspase-3阳性或caspase-3高表达或两者结合与DFS差相关。有趣的是，分层分析显示，阳性切割的半胱天冬酶-3或/和更高的半胱天冬酶-3的共表达水平与疾病晚期患者的更好的疾病特异性生存相关，例如肿瘤尺寸大和淋巴结浸润，而它与中等细胞分化和小肿瘤尺寸的OTSCC患者的差DFS相关。总之，裂解的caspase-3和caspase-3/8/9可以作为OTSCC患者肿瘤发生的生物标志物。切割的caspase-3和caspase-3的共表达水平可能是OTSCC患者的预后生物标志物，特别是在具有某些肿瘤阶段和细胞分化状态的那些患者中。
Apoptosis plays a dual role in cancer development and malignancy. The role of apoptosis-related caspases in cancer remains controversial, particularly in oral tongue squamous cell carcinoma (OTSCC). In this study, we examined the protein levels of cleaved caspase-3, caspase-3, caspase-8, and caspase-9 on tissue microarrays consisting of samples from 246 OTSCC patients by immunohistochemistry. Wilcoxon signed-rank test indicated that the protein levels of cleaved caspase-3, caspase-3, caspase-8, and caspase-9 in tumor tissues were significantly higher compared to those in adjacent normal tissues (all p<0.001). The expression level of caspase-8 in tumors was elevated in patients with lymph node invasion. Moreover, positive expression of cleaved caspase-3 was associated with shorter disease-free survival (DFS) in OTSCC patients with moderate differentiation and lymph node invasion. Combination of either positive cleaved caspase-3 or higher caspase-3 expression or both was associated with poor DFS. Interestingly, stratification analysis showed that co-expression levels of positive cleaved caspase-3 or/and higher caspase-3 were associated with better disease-specific survival in patients with advanced stages of the disease, such as large tumor size and lymph node invasion, whereas it was associated with poor DFS in OTSCC patients with moderate cell differentiation and small tumor size. Taken together, cleaved caspase-3 and caspase-3/8/9 could be biomarkers for tumorigenesis in OTSCC patients. The co-expression level of cleaved caspase-3 and caspase-3 might be a prognostic biomarker for OTSCC patients, particular in those patients with certain tumor stages and cell differentiation status.
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影响因子：
7.2
作者：
McIlwain, David R.;Berger, Thorsten;Mak, Tak W.
通讯作者：
Mak, Tak W.
影响因子：
9
作者：
Nakanishi, Masako;Rosenberg, Daniel W.
通讯作者：
Rosenberg, Daniel W.
BAG3 通过减弱自噬促进饥饿诱导的甲状腺癌细胞凋亡
影响因子：
5.8
作者：
Li, Si;Zhang, Hai-Yan;Du, Zhen-Xian
通讯作者：
Du, Zhen-Xian
DOI：
10.1002/hed.21602
发表时间：
2011-08-01
影响因子：
2.9
作者：
Coutinho-Camillo, Claudia Malheiros;Lourenco, Silvia Vanessa;Soares, Fernando Augusto
通讯作者：
Soares, Fernando Augusto
影响因子：
7.2
作者：
Ryoo HD;Bergmann A
通讯作者：
Bergmann A