Organic Matter Regulates Ammonia-Oxidizing Bacterial and Archaeal Communities in the Surface Sediments of Ctenopharyngodon idellus Aquaculture Ponds.
Organic Matter Regulates Ammonia-Oxidizing Bacterial and Archaeal Communities in the Surface Sediments of Ctenopharyngodon idellus Aquaculture Ponds.
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DOI：
10.3389/fmicb.2018.02290
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发表时间：
2018
影响因子：
5.2
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Dai L;Liu C;Yu L;Song C;Peng L;Li X;Tao L;Li G
氨氧化菌（AOB）和古菌（AOA）在水产养殖池塘脱氮中发挥重要作用，但其分布及影响其分布的环境因素尚不清楚。本研究采集了中国三个不同养殖区的草鱼池塘表层沉积物样品。对池塘中氨氧化细菌和氨氧化细菌的群落结构及理化特性进行了研究。结果表明，除一个采样池外，其他采样池AOA含量均高于AOB，但不同采样池底泥AOB和AOA含量差异较大。相关分析表明，AOB和芳基硫酸酯酶的丰度之间的显着相关性，以及AOA的丰度和总氮（TN）和芳基硫酸酯酶。AOB/AOA比值与微生物量碳显著相关。AOB分为亚硝化螺旋菌属（Nitrosospira）和亚硝化单胞菌属（Nitrosomonas）的7个类群，AOA分为亚硝化球菌属（Nitrososphaera）、亚硝化球菌属（Nitrososphaera）姐妹类群和亚硝化微菌属（Nitrosopumilus）的6个类群。水产养殖池塘表层沉积物中AOB/AOA多样性随总有机碳（TOC）水平而变化，AOB和AOA多样性分别与芳基硫酸酯酶和β-葡萄糖苷酶呈显著相关。AOB群落的组成也被发现是显着影响沉积物富营养化状态（TOC和TN水平），和pH值。此外，酸性磷酸酶和芳基硫酸酯酶在表层沉积物中的浓度显着相关的突出细菌amoA基因型，和TOC和脲酶的浓度被发现显着相关的突出古细菌amoA基因型组合物。综上所述，我们的研究结果表明，草鱼养殖池塘表层沉积物中AOB和AOA群落受有机质及其对微生物的可利用性的调节。
Ammonia-oxidizing bacteria (AOB) and archaea (AOA) play important roles in nitrogen removal in aquaculture ponds, but their distribution and the environmental factors that drive their distribution are largely unknown. In this study, we collected surface sediment samples from Ctenopharyngodon idellus ponds in three different areas in China that practice aquaculture. The community structure of AOB and AOA and physicochemical characteristics in the ponds were investigated. The results showed that AOA were more abundant than AOB in all sampling ponds except one, but sediment AOB and AOA numbers varied greatly between ponds. Correlation analyses indicated a significant correlation between the abundance of AOB and arylsulfatase, as well as the abundance of AOA and total nitrogen (TN) and arylsulfatase. In addition, AOB/AOA ratio was found to be significantly correlated with the microbial biomass carbon. AOB were grouped into seven clusters affiliated to Nitrosospira and Nitrosomonas, and AOA were grouped into six clusters affiliated to Nitrososphaera, Nitrososphaera sister group, and Nitrosopumilus. AOB/AOA diversity in the surface sediments of aquaculture ponds varied according to the levels of total organic carbon (TOC), and AOB and AOA diversity was significantly correlated with arylsulfatase and β-glucosidase, respectively. The compositions of the AOB communities were also found to be significantly influenced by sediment eutrophic status (TOC and TN levels), and pH. In addition, concentrations of acid phosphatase and arylsulfatase in surface sediments were significantly correlated with the prominent bacterial amoA genotypes, and concentrations of TOC and urease were found to be significantly correlated with the prominent archaeal amoA genotype compositions. Taken together, our results indicated that AOB and AOA communities in the surface sediments of Ctenopharyngodon idellus aquaculture ponds are regulated by organic matter and its availability to the microorganisms.
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影响因子：
4.4
作者：
Beman, J. Michael;Francis, Christopher A.
通讯作者：
Francis, Christopher A.
影响因子：
5.2
作者：
Kitidis, Vassilis;Laverock, Bonnie;Widdicombe, Stephen
通讯作者：
Widdicombe, Stephen
影响因子：
2.1
作者：
Kurola, J;Salkinoja-Salonen, M;Romantschuk, M
通讯作者：
Romantschuk, M
影响因子：
9.7
作者：
Bandick, AK;Dick, RP
通讯作者：
Dick, RP
北运河沉积物中氨氧化微生物的丰度及分布
影响因子：
3
作者：
Bao, Linlin;Wang, Xiaoyan;Chen, Yongjuan
通讯作者：
Chen, Yongjuan