Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry.
Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry.
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DOI：
10.1149/2.0091604jes
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发表时间：
2016
影响因子：
3.9
通讯作者：
Shen M
中科院分区：
文献类型：
--
作者：
Colombo ML;McNeil S;Iwai N;Chang A;Shen M
我们在这里展示了在半径为数百纳米的纳米吸管电极上检测多巴胺（DA），范围从160纳米到480纳米。二苯并-18-冠-6 （DB18C6）作为离子载体促进DA转移，得到半波转移电位E1/2， DA比E1/2， TBA为−0.322（±0.020）V。在DA的转移过程中，观察到明确的稳态s型循环伏安图。采用高分辨率扫描电镜对纳米吸管电极的尺寸和锥度角进行了测量。DA浓度在0.25 mM ~ 2mm范围内呈线性；在上述尺寸的纳米管电极上计算得到的扩散系数为4.87（±0.28）× 10−10 m2/s。研究了抗坏血酸对纳米电极检测DA的影响，发现DA检测仍然表现为线性行为，具有明确的s型cv， DA为- 0.328（±0.029）V vs E1/2， TBA。在半径为161 ~ 263 nm的纳米电极上，测定了2 mM AA存在时，DA在MgCl2中的扩散系数为1.93（±0.59）× 10−10 m2/s，而0.1 mM AA的生理浓度对DA的扩散系数没有影响。
We present here the detection of dopamine (DA) at nanopipet electrodes with radii of hundreds of nanometers ranging from 160 nm to 480 nm. Dibenzo-18-crown-6 (DB18C6) was employed as an ionophore to facilitate DA transfer, resulting in a half-wave transfer potential, E1/2, DA, of −0.322 (±0.020) V vs. E1/2, TBA. Well-defined steady-state sigmoidal cyclic voltammograms were observed for the transfer of DA. High resolution scanning electron microscopy was used to measure the size and taper angle of the nanopipet electrodes. The detection is linear with concentration of DA ranging from 0.25 mM to 2 mM; calculated diffusion coefficient at nanopipet electrodes with above mentioned sizes is 4.87 (±0.28) × 10−10 m2/s. The effect of the common interferent ascorbic acid on DA detection with nanopipet electrodes was evaluated, where DA detection still shows linear behavior with well-defined sigmoidal CVs with E1/2, DA being −0.328 (±0.029) V vs. E1/2, TBA. The diffusion coefficient for DA transfer in MgCl2 with the presence of 2 mM AA was measured to be 1.93 (±0.59) × 10−10 m2/s on nanoelectrodes with radii from 161 nm to 263 nm, but the physiological concentration of 0.1 mM AA had no effect on DA’s diffusion coefficient.
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影响因子：
64.8
作者：
DETOLEDO, GA;FERNANDEZCHACON, R;FERNANDEZ, JM
通讯作者：
FERNANDEZ, JM
影响因子：
4.9
作者：
Arrigan, DWM;Ghita, M;Beni, V
通讯作者：
Beni, V
影响因子：
3.3
作者：
Cousins, MS;Salamone, JD
通讯作者：
Salamone, JD
影响因子：
7.4
作者：
Colombo, Michelle L.;Sweedler, Jonathan V.;Shen, Mei
通讯作者：
Shen, Mei
影响因子：
6.2
作者：
Berduque, Alfonso;Zazpe, Raul;Arrigan, Damien W. M.
通讯作者：
Arrigan, Damien W. M.