Nanopipet-Based Liquid-Liquid Interface Probes for the Electrochemical Detection of Acetylcholine, Tryptamine, and Serotonin via Ionic Transfer.
Nanopipet-Based Liquid-Liquid Interface Probes for the Electrochemical Detection of Acetylcholine, Tryptamine, and Serotonin via Ionic Transfer.
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DOI：
10.1021/ac504151e
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发表时间：
2015
影响因子：
7.4
通讯作者：
Shen, Mei
中科院分区：
文献类型：
--
作者：
Colombo, Michelle L.;Sweedler, Jonathan V.;Shen, Mei
两种不混溶的电解质溶液（ITIES）之间的纳米级界面提供了一个独特的分析平台，用于检测生物感兴趣的离子物种，例如神经递质和神经调质，特别是那些在没有电极修饰的情况下难以直接在碳电极上检测的离子物种。我们报告的检测乙酰胆碱，血清素，色胺纳米吸量管电极探针的大小范围从半径为107至35 nm。通过循环伏安法和安培法研究了这些分析物在1，2-二氯乙烷/水界面上的迁移。在人工海水的电位窗口内，在纳米管电极上观察到乙酰胆碱和色胺的定义良好的S形伏安图。乙酰胆碱、色胺和5-羟色胺的半波转移电位E1/2分别为−0.11、−0.25和−0.47 V（相对于E1/2，TEA）（该术语在实验中定义）。人工海水中乙酰胆碱和色胺的线性范围分别为0.25-6 mM和0.5-10 mM。5-羟色胺的转移在LiCl溶液中在0.15-8 mM范围内呈线性。在半径为21 nm的纳米吸量管电极上，氯化锂中5-羟色胺的检测限为77 μM，在半径为17 nm的纳米吸量管电极上，乙酰胆碱的检测限为205 μM，在半径为19 nm的纳米吸量管电极上，色胺的检测限为86 μM。纳米移液管支持的ITIES探针具有很大的潜力，可用于纳米空间分辨率测量，用于检测神经递质。
A nanoscale interface between two immiscible electrolyte solutions (ITIES) provides a unique analytical platform for the detection of ionic species of biological interest such as neurotransmitters and neuromodulators, especially those that are otherwise difficult to detect directly on a carbon electrode without electrode modification. We report the detection of acetylcholine, serotonin, and tryptamine on nanopipet electrode probes with sizes ranging from a radius of ≈7 to 35 nm. The transfer of these analytes across a 1,2-dichloroethane/water interface was studied by cyclic voltammetry and amperometry. Well-defined sigmoidal voltammograms were observed on the nanopipet electrodes within the potential window of artificial seawater for acetylcholine and tryptamine. The half wave transfer potential, E1/2, of acetylcholine, tryptamine, and serotonin were found to be −0.11, −0.25, and −0.47 V vs E1/2,TEA (term is defined later in experimental), respectively. The detection was linear in the range of 0.25–6 mM for acetylcholine and of 0.5–10 mM for tryptamine in artificial seawater. Transfer of serotonin was linear in the range of 0.15–8 mM in LiCl solution. The limit of detection for serotonin in LiCl on a radius ≈21 nm nanopipet electrode was 77 μM, for acetylcholine on a radius ≈7 nm nanopipet electrode was 205 μM, and for tryptamine on a radius ≈19 nm nanopipet electrode was 86 μM. Nanopipet-supported ITIES probes have great potential to be used in nanometer spatial resolution measurements for the detection of neurotransmitters.
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影响因子：
64.8
作者：
DETOLEDO, GA;FERNANDEZCHACON, R;FERNANDEZ, JM
通讯作者：
FERNANDEZ, JM
影响因子：
4.9
作者：
Arrigan, DWM;Ghita, M;Beni, V
通讯作者：
Beni, V
影响因子：
64.8
作者：
CHOW, RH;VONRUDEN, L;NEHER, E
通讯作者：
NEHER, E
影响因子：
--
作者：
Aidoo, Anthony Y.;Ward, Kim
通讯作者：
Ward, Kim
影响因子：
64.8
作者：
BRUNS, D;JAHN, R
通讯作者：
JAHN, R