Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes.
Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes.
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DOI：
10.1016/j.fob.2012.08.005
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发表时间：
2012
期刊：
影响因子：
2.6
通讯作者：
Shirasawa S
中科院分区：
文献类型：
--
作者：
Matsuzaki H;Fujimoto T;Ota T;Ogawa M;Tsunoda T;Doi K;Hamabashiri M;Tanaka M;Shirasawa S
Tespa 1最近被报道是T细胞发育中的关键分子，然而，Tespa 1的精确分子机制仍然难以捉摸。在这里，我们证明，Tespa 1显示氨基酸序列同源性KRAS诱导肌动蛋白相互作用蛋白（KRAP），肌醇1，4，5-三磷酸受体（IP 3 R）结合蛋白，和Tespa 1物理协会与IP 3 R在T和B淋巴细胞。Tespa 1中的两个连续的苯丙氨酸残基（Phe 185/Phe 186）在Tespa 1和KRAP之间是保守的，对于Tespa 1和IP 3R之间的关联是必不可少的。这些发现表明，Tespa 1通过调节IP 3R在免疫系统中起着关键作用。我们确定Tespa 1是淋巴组织中一种新的IP 3R相关蛋白。Tespa 1与T和B淋巴细胞中的多种IP 3 R亚型相互作用。IP 3R的氨基末端区域足以与Tespa 1结合。Tespa 1中的两个连续的苯丙氨酸对于与IP 3R的相互作用至关重要。Tespa 1在结构和功能上与KRAP相关。
Tespa1 has been recently reported to be a critical molecule in T-cell development, however, the precise molecular mechanisms of Tespa1 remain elusive. Here, we demonstrate that Tespa1 shows amino-acid sequence homology to KRAS-induced actin-interacting protein (KRAP), an inositol 1,4,5-trisphosphate receptor (IP3R) binding protein, and that Tespa1 physically associates with IP3R in T and B lymphocytes. Two-consecutive phenylalanine residues (Phe185/Phe186) in Tespa1, which are conserved between Tespa1 and KRAP, are indispensable for the association between Tespa1 and IP3R. These findings suggest that Tespa1 plays critical roles in the immune system through the regulation of the IP3R. ▸ We identified Tespa1 as a novel IP3R-associated protein in lymphoid tissues. ▸ Tespa1 interacts with multiple subtypes of IP3R in T and B lymphocytes. ▸ Amino-terminal region of IP3R is sufficient for the association with Tespa1. ▸ Two consecutive phenylalanines in Tespa1 are critical for the interaction with IP3R. ▸ Tespa1 is structurally and functionally related to KRAP.
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影响因子：
3.5
作者：
SUGIYAMA, T;YAMAMOTOHINO, M;HASEGAWA, M
通讯作者：
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DOI：
10.1083/jcb.200708200
发表时间：
2007-12-03
期刊：
The Journal of cell biology
影响因子：
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作者：
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DOI：
10.1016/j.bbrc.2011.04.016
发表时间：
2011-05-06
影响因子：
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作者：
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Shirasawa, Senji
DOI：
10.1073/pnas.89.10.4265
发表时间：
1992-05-15
影响因子：
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作者：
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通讯作者：
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