The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice.
The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice.
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DOI：
10.1016/j.vaccine.2005.01.036
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发表时间：
2005-03-18
期刊：
影响因子：
5.5
通讯作者：
Sakatani M
中科院分区：
文献类型：
--
作者：
Okada M;Takemoto Y;Okuno Y;Hashimoto S;Yoshida S;Fukunaga Y;Tanaka T;Kita Y;Kuwayama S;Muraki Y;Kanamaru N;Takai H;Okada C;Sakaguchi Y;Furukawa I;Yamada K;Matsumoto M;Kase T;Demello DE;Peiris JS;Chen PJ;Yamamoto N;Yoshinaka Y;Nomura T;Ishida I;Morikawa S;Tashiro M;Sakatani M
我们已经研究了使用编码结构抗原的cDNA构建体来开发针对SARS CoV的新型疫苗;来自SARS CoV的刺突蛋白（S）、膜蛋白（M）、包膜蛋白（E）或核衣壳（N）蛋白。用pcDNA 3.1（+）质粒载体接种SARS-N或-M DNA的小鼠分别产生针对N或M蛋白的T细胞免疫应答（CTL诱导和增殖）。同时检测到对SARS DNA转染的Ⅱ型肺泡上皮细胞（T7细胞克隆）的CTL应答，这些细胞被认为是SARS病毒感染人类的初始靶细胞。为了确定这些DNA疫苗是否可以在人类以及小鼠中诱导T细胞免疫应答，用这些DNA疫苗免疫SCID-PBL/hu小鼠。如预期的，从人T细胞诱导病毒特异性CTL应答和T细胞增殖。SARS-N和SARS-M DNA疫苗和SCID-PBL/hu小鼠模型在保护性疫苗的研制中具有重要意义。
We have investigated to develop novel vaccines against SARS CoV using cDNA constructs encoding the structural antigen; spike protein (S), membrane protein (M), envelope protein (E), or nucleocapsid (N) protein, derived from SARS CoV. Mice vaccinated with SARS-N or -M DNA using pcDNA 3.1(+) plasmid vector showed T cell immune responses (CTL induction and proliferation) against N or M protein, respectively. CTL responses were also detected to SARS DNA-transfected type II alveolar epithelial cells (T7 cell clone), which are thought to be initial target cells for SARS virus infection in human. To determine whether these DNA vaccines could induce T cell immune responses in humans as well as in mice, SCID-PBL/hu mice was immunized with these DNA vaccines. As expected, virus-specific CTL responses and T cell proliferation were induced from human T cells. SARS-N and SARS-M DNA vaccines and SCID-PBL/hu mouse model will be important in the development of protective vaccines.
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影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
DOI：
10.1073/pnas.78.12.7717
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
OKADA, M;YOSHIMURA, N;KISHIMOTO, T
通讯作者：
KISHIMOTO, T
DNA疫苗可诱导小鼠中和冠状病毒中和和保护性免疫。
影响因子：
64.8
作者：
Yang ZY;Kong WP;Huang Y;Roberts A;Murphy BR;Subbarao K;Nabel GJ
通讯作者：
Nabel GJ
DOI：
10.1016/s0140-6736(03)14962-8
发表时间：
2003-12-06
期刊：
Lancet (London, England)
影响因子：
--
作者：
Gao W;Tamin A;Soloff A;D'Aiuto L;Nwanegbo E;Robbins PD;Bellini WJ;Barratt-Boyes S;Gambotto A
通讯作者：
Gambotto A