Characterization of cell cycle specific protein interaction networks of the yeast 26S proteasome complex by the QTAX strategy.
Characterization of cell cycle specific protein interaction networks of the yeast 26S proteasome complex by the QTAX strategy.
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DOI：
10.1021/pr1000175
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发表时间：
2010-04-05
影响因子：
4.4
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Kaake RM;Milenković T;Przulj N;Kaiser P;Huang L
泛素-蛋白酶体依赖的蛋白质降解在真核细胞周期调控中起着重要作用。不同阶段之间的细胞周期转换受到严格调节，以防止不受控制的细胞增殖，这是癌细胞的特征。为了解26 S蛋白酶体在细胞周期中的时相特异性调控，揭示泛素-蛋白酶体降解途径在细胞周期中的分子机制，我们采用QTAX分析方法对同步化酵母细胞中的细胞周期时相特异性蛋白酶体相互作用蛋白（PIP）进行了全面的表征。我们的努力已经产生了特定的蛋白酶体相互作用网络的G1，S和M期的细胞周期，并确定了共677 PIP，其中266个以前没有确定从非同步化细胞。在动态变化的基础上，他们的SILAC比率在三个细胞周期阶段，我们采用了一个档案矢量为基础的聚类方法，并确定了20个功能显着的PIP组，其中3个是丰富的细胞周期相关的功能。这项工作是了解动态蛋白酶体相互作用如何参与细胞周期中的各种细胞途径的第一步。
Ubiquitin-proteasome dependent protein degradation plays a fundamental role in the regulation of the eukaryotic cell cycle. Cell cycle transitions between different phases are tightly regulated to prevent uncontrolled cell proliferation, which is characteristic of cancer cells. To understand cell cycle phase specific regulation of the 26S proteasome and reveal the molecular mechanisms underlying the ubiquitin-proteasome degradation pathway during cell cycle progression, we have carried out comprehensive characterization of cell cycle phase specific proteasome interacting proteins (PIPs) by QTAX analysis of synchronized yeast cells. Our efforts have generated specific proteasome interaction networks for the G1, S, and M phases of the cell cycle and identified a total of 677 PIPs, 266 of which were not previously identified from unsynchronized cells. On the basis of the dynamic changes of their SILAC ratios across the three cell cycle phases, we have employed a profile vector-based clustering approach and identified 20 functionally significant groups of PIPs, 3 of which are enriched with cell cycle related functions. This work presents the first step toward understanding how dynamic proteasome interactions are involved in various cellular pathways during the cell cycle.
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影响因子：
4.4
作者：
Fang L;Wang X;Yamoah K;Chen PL;Pan ZQ;Huang L
通讯作者：
Huang L
DOI：
10.1083/jcb.141.3.567
发表时间：
1998-05-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Brizzio V;Gammie AE;Rose MD
通讯作者：
Rose MD
影响因子：
16
作者：
Cho, RJ;Campbell, MJ;Davis, RW
通讯作者：
Davis, RW
DOI：
10.1196/annals.1402.078
发表时间：
2007-01-01
期刊：
SKELETAL BIOLOGY AND MEDICINE, PT A
影响因子：
--
作者：
Ciechanover, Aaron
通讯作者：
Ciechanover, Aaron
影响因子：
16.6
作者：
Finley D
通讯作者：
Finley D