A strategy for stimulating astaxanthin and lipid production in Haematococcus pluvialis by exogenous glycerol application under low light
A strategy for stimulating astaxanthin and lipid production in Haematococcus pluvialis by exogenous glycerol application under low light
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弱光下应用外源甘油刺激雨生红球藻虾青素和脂质产生的策略
DOI：
10.1016/j.algal.2019.101779
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发表时间：
2020-03
期刊：
影响因子：
--
通讯作者：
Yang Na
中科院分区：
文献类型：
--
作者：
Zhang Litao;Zhang Chunhui;Liu Jianguo;Yang Na
在大规模的室外栽培中，雨生红球藻培养物经常暴露于由连续降雨条件引起的低光下。弱光显著限制生物量和虾青素积累，从而降低虾青素生产率。研究了弱光条件下外源甘油对雨生红球藻（H. pluvialis）生长、光合作用、虾青素和脂质积累的影响。研究了在弱光条件下应用外源甘油促进雨生红球藻（H. pluvialis）产虾青素和脂质的策略。在弱光条件下，外源甘油并没有改变雨生红球藻的生物量和光合能力。在11天的生长期内，添加1 mL L-1甘油通过增加脂肪酸的形成使总脂质含量增加了36.8%。此外，外源甘油可以通过增加底物（如丙酮酸）的形成来直接促进弱光下虾青素的产生。同时，外源甘油的加入提高了脂肪酸水平，促进了虾青素的酯化反应，从而间接促进了虾青素的生物合成，这一点在弱光下表现为外源甘油显著提高了虾青素酯的含量。这些结果表明雨生红球藻在弱光条件下具有利用甘油生产虾青素和脂质的能力。
In large-scale outdoor cultivation, Haematococcus pluvialis cultures are frequently exposed to low light caused by continuous rain conditions. Low light significantly restricts biomass and astaxanthin accumulation, thereby decreasing astaxanthin productivity. In this study, the effects of exogenous glycerol on the growth, photosynthesis, and astaxanthin and lipid accumulation inH.pluvialisunder low light were investigated. A strategy was developed for promoting astaxanthin and lipid production inH.pluvialisunder low light by the application of exogenous glycerol. Under low light, exogenous glycerol did not change the biomass and photosynthetic capacity ofH.pluvialis. The addition of 1 mL L−1glycerol increased total lipid content by 36.8% by increasing the formation of fatty acids during an 11-day growth period. Furthermore, exogenous glycerol could directly accelerate astaxanthin production under low light by increasing the formation of substrates (e.g. pyruvate). Meanwhile, the elevated fatty acid level due to the addition of glycerol facilitated the esterification of astaxanthin, thereby accelerating the biosynthesis of astaxanthin indirectly, which is indicated by the fact that exogenous glycerol enhanced astaxanthin esters content significantly under low light. All these results suggest thatH.pluvialishas the ability to utilize glycerol for astaxanthin and lipid production under low light.
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DOI：
10.1016/j.plaphy.2016.05.029
发表时间：
2016-10
期刊：
Plant physiology and biochemistry : PPB
影响因子：
--
作者：
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影响因子：
13.6
作者：
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DOI：
10.1016/j.algal.2018.05.021
发表时间：
2018-07-01
影响因子：
5.1
作者：
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