Real-time immune cell interactions in target tissue during autoimmune-induced damage and graft tolerance.
Real-time immune cell interactions in target tissue during autoimmune-induced damage and graft tolerance.
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DOI：
10.1084/jem.20130785
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发表时间：
2014-03-10
期刊：
影响因子：
--
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Miska J;Abdulreda MH;Devarajan P;Lui JB;Suzuki J;Pileggi A;Berggren PO;Chen Z
自身免疫诱导的组织损伤和Treg细胞保护的活体可视化显示了胰岛移植物中基于接触的免疫细胞相互作用和旁观者组织细胞的生长。实时成像研究正在重塑免疫学范式，但在靶组织中的效应期缺乏自身抗原特异性T细胞的视觉框架。为了解决这个问题，我们进行了活体，纵向成像分析的细胞行为在非淋巴靶组织，以说明T细胞生物学的一些关键方面。我们使用T细胞介导的损伤和胰岛移植物保护的小鼠模型。CD4+和CD8+效应T（Teff）淋巴细胞直接参与靶细胞。引人注目的是，缺乏特异性抗原的并列β细胞不受旁观者破坏，而是在几天内大量生长，可能是通过复制。在靶组织中，Foxp3+调节性T（Treg）细胞持续接触Teff细胞，有或没有CD11c+树突状细胞的参与，这一观察结果与Treg功能的体外“商标”接触依赖性抑制相一致。这项研究阐明了通过靶组织中基于接触的免疫细胞相互作用诱导耐受，并强调了在炎症环境中抗原隐匿下组织再生的潜力。
Intravital visualization of autoimmune-induced tissue damage and Treg cell protection shows contact-based immune cell interactions and growth of bystander tissue cells in pancreatic islet grafts. Real-time imaging studies are reshaping immunological paradigms, but a visual framework is lacking for self-antigen-specific T cells at the effector phase in target tissues. To address this issue, we conducted intravital, longitudinal imaging analyses of cellular behavior in nonlymphoid target tissues to illustrate some key aspects of T cell biology. We used mouse models of T cell–mediated damage and protection of pancreatic islet grafts. Both CD4+ and CD8+ effector T (Teff) lymphocytes directly engaged target cells. Strikingly, juxtaposed β cells lacking specific antigens were not subject to bystander destruction but grew substantially in days, likely by replication. In target tissue, Foxp3+ regulatory T (Treg) cells persistently contacted Teff cells with or without involvement of CD11c+ dendritic cells, an observation conciliating with the in vitro “trademark” of Treg function, contact-dependent suppression. This study illustrates tolerance induction by contact-based immune cell interaction in target tissues and highlights potentials of tissue regeneration under antigenic incognito in inflammatory settings.
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影响因子：
4.4
作者：
Baecher-Allan, Clare;Wolf, Elizabeth;Haller, David A.
通讯作者：
Haller, David A.
DOI：
10.1073/pnas.0607854103
发表时间：
2006-10-31
影响因子：
11.1
作者：
Chen, Zhibin;Stockton, John;Benoist, Christophe
通讯作者：
Benoist, Christophe
DOI：
10.1073/pnas.1105002108
发表时间：
2011-08-02
影响因子：
11.1
作者：
Abdulreda, Midhat H.;Faleo, Gaetano;Berggren, Per-Olof
通讯作者：
Berggren, Per-Olof
影响因子：
64.8
作者：
Dor, Y;Brown, J;Melton, DA
通讯作者：
Melton, DA
DOI：
10.4049/jimmunol.1101027
发表时间：
2012-02-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Huang YH;Sojka DK;Fowell DJ
通讯作者：
Fowell DJ