Ultrastructure of GABA- and Tachykinin-Immunoreactive Neurons in the Lower Division of the Central Body of the Desert Locust.
Ultrastructure of GABA- and Tachykinin-Immunoreactive Neurons in the Lower Division of the Central Body of the Desert Locust.
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DOI：
10.3389/fnbeh.2016.00230
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发表时间：
2016
影响因子：
3
通讯作者：
Müller M
中科院分区：
文献类型：
--
作者：
Homberg U;Müller M
中央复合体是一组横跨昆虫大脑中线的神经丸，在空间定位和导航中起着关键作用。在沙漠蝗和其他物种中，中央复合体的许多神经元对动物上方偏振光的振荡面很敏感，并且可能参与了从天空偏振模式中获得的罗盘方向的编码。偏振光信号主要通过两种γ-氨基丁酸（GABA）免疫反应的切向神经元TL2和TL3进入蝗虫中央复合体，它们支配中央体（CBL）下层的特定层。候选的突触后伙伴是连接CBL和原脑桥（PB）的柱状神经元（CL1）。CL1神经元亚群对抗血小板收缩素（LomTK）血清具有免疫反应性。为了更好地了解CBL中切向和柱状神经元的突触连通性，我们研究了沙漠蝗CBL的超微结构组织，采用常规电镜和免疫标记GABA或LomTK的制剂。CBL内的神经元分布有丰富的线粒体和囊泡。结果表明：小透明囊泡（直径20 ~ 40 nm）、暗密核囊泡（直径70 ~ 120 nm）、粒状密核囊泡（直径70 ~ 80 nm）。神经元通过发散的二联体连接，偶尔也通过收敛的二联体连接。gaba免疫反应神经元含有小而透明的囊泡和少量暗而致密的核心囊泡。他们有突触前和突触后的接触，但输出突触比输入突触更频繁地被观察到。LomTK免疫染色集中于大颗粒囊泡；神经元具有突触前和突触后的连接，通常被认为是gaba能神经元。数据表明，gaba免疫反应的切向神经元向CBL突触后神经元提供信号，包括lomtk免疫标记的CL1神经元，但也接受来自lomtk标记的神经元的输入。这两种类型的神经元都与CBL的其他成分一起参与局部回路。
The central complex, a group of neuropils spanning the midline of the insect brain, plays a key role in spatial orientation and navigation. In the desert locust and other species, many neurons of the central complex are sensitive to the oscillation plane of polarized light above the animal and are likely involved in the coding of compass directions derived from the polarization pattern of the sky. Polarized light signals enter the locust central complex primarily through two types of γ-aminobutyric acid (GABA)-immunoreactive tangential neurons, termed TL2 and TL3 that innervate specific layers of the lower division of the central body (CBL). Candidate postsynaptic partners are columnar neurons (CL1) connecting the CBL to the protocerebral bridge (PB). Subsets of CL1 neurons are immunoreactive to antisera against locustatachykinin (LomTK). To better understand the synaptic connectivities of tangential and columnar neurons in the CBL, we studied its ultrastructural organization in the desert locust, both with conventional electron microscopy and in preparations immunolabeled for GABA or LomTK. Neuronal profiles in the CBL were rich in mitochondria and vesicles. Three types of vesicles were distinguished: small clear vesicles with diameters of 20–40 nm, dark dense-core vesicles (diameter 70–120 nm), and granular dense-core vesicles (diameter 70–80 nm). Neurons were connected via divergent dyads and, less frequently, through convergent dyads. GABA-immunoreactive neurons contained small clear vesicles and small numbers of dark dense core vesicles. They had both pre- and postsynaptic contacts but output synapses were observed more frequently than input synapses. LomTK immunostaining was concentrated on large granular vesicles; neurons had pre- and postsynaptic connections often with neurons assumed to be GABAergic. The data suggest that GABA-immunoreactive tangential neurons provide signals to postsynaptic neurons in the CBL, including LomTK-immunolabeled CL1 neurons, but in addition also receive input from LomTK-labeled neurons. Both types of neuron are additionally involved in local circuits with other constituents of the CBL.
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影响因子：
5.3
作者：
Heinze, Stanley;Gotthardt, Sascha;Homberg, Uwe
通讯作者：
Homberg, Uwe
影响因子：
16.2
作者：
Heinze, Stanley;Reppert, Steven M.
通讯作者：
Reppert, Steven M.
影响因子：
2.5
作者：
Homberg, U;Hofer, S;Gebhardt, S
通讯作者：
Gebhardt, S
DOI：
10.1007/bf00271538
发表时间：
1991-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
MALUN, D
通讯作者：
MALUN, D
影响因子：
3.6
作者：
HOMBERG, U;KINGAN, TG;HILDEBRAND, JG
通讯作者：
HILDEBRAND, JG