N-Terminal Processing and Modification of Ciliary Dyneins.
N-Terminal Processing and Modification of Ciliary Dyneins.
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作者：
轴线动力蛋白是一种高度复杂的微管马达，为纤毛运动提供动力。这些多亚单位酶在细胞质内的特定位置组装。至少需要19种胞浆因子来产生动力蛋白全酶和/或将它们运输到生长中的纤毛。许多蛋白质经过N-末端的加工和乙酰化，可以产生服从ACN-末端规则的简并，改变N-末端的静电，产生新的结合界面，并通过靶向降解影响亚单位的化学计量。在这里，我们使用纤毛样品和从衣藻中电泳纯化的动力蛋白重链的质谱学来确定它们的N-末端加工；我们还详细地描述了这种生物中存在的N-末端乙酰酶复合体。我们通过两种不同的乙酰酶根据动力蛋白重链的加工途径识别出四种类型的动力蛋白重链，其中一种依赖于蛋氨酸氨基肽酶的活性。此外，我们还发现，动力蛋白外臂中间/轻链亚基和对接复合体的一个组分被加工成未经修饰的Pro残基，这可能为指导其他含有N-末端降解的动力蛋白复合体亚单位的胞液化学计量学提供了一个设定点。因此，我们识别和描述了导致细胞质中轴丝动力蛋白形成的途径的额外水平的加工和复杂性。
Axonemal dyneins are highly complex microtubule motors that power ciliary motility. These multi-subunit enzymes are assembled at dedicated sites within the cytoplasm. At least nineteen cytosolic factors are specifically needed to generate dynein holoenzymes and/or for their trafficking to the growing cilium. Many proteins are subject to N-terminal processing and acetylation, which can generate degrons subject to the AcN-end rule, alter N-terminal electrostatics, generate new binding interfaces, and affect subunit stoichiometry through targeted degradation. Here, we have used mass spectrometry of cilia samples and electrophoretically purified dynein heavy chains from Chlamydomonas to define their N-terminal processing; we also detail the N-terminal acetylase complexes present in this organism. We identify four classes of dynein heavy chain based on their processing pathways by two distinct acetylases, one of which is dependent on methionine aminopeptidase activity. In addition, we find that one component of both the outer dynein arm intermediate/light chain subcomplex and the docking complex is processed to yield an unmodified Pro residue, which may provide a setpoint to direct the cytosolic stoichiometry of other dynein complex subunits that contain N-terminal degrons. Thus, we identify and describe an additional level of processing and complexity in the pathways leading to axonemal dynein formation in cytoplasm.
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衣原体鞭毛再生期间特定鞭毛蛋白的合成，运输和利用。
影响因子：
7.8
作者：
Remillard, S P;Witman, G B
通讯作者：
Witman, G B
DOI：
10.1083/jcb.112.3.441
发表时间：
1991-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kamiya R;Kurimoto E;Muto E
通讯作者：
Muto E
影响因子：
4
作者：
Aksnes，Henriette;McTiernan，Nina;Arnesen，Thomas
通讯作者：
Arnesen，Thomas
影响因子：
12.7
作者：
Faubel, Regina J.;Canellas, Veronica S. Santos;Lo, Cecilia W.
通讯作者：
Lo, Cecilia W.
DOI：
10.1083/jcb.200903066
发表时间：
2009-08-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Huang K;Diener DR;Rosenbaum JL
通讯作者：
Rosenbaum JL