The ubiquitin conjugation system is involved in the disassembly of cilia and flagella.
The ubiquitin conjugation system is involved in the disassembly of cilia and flagella.
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DOI：
10.1083/jcb.200903066
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发表时间：
2009-08-24
期刊：
影响因子：
--
通讯作者：
Rosenbaum JL
中科院分区：
文献类型：
--
作者：
Huang K;Diener DR;Rosenbaum JL
纤毛和鞭毛的脱落与细胞周期和环境因素有关。我们发现鞭毛蛋白的泛素化是鞭毛分解的一个组成部分。鞭毛中检测到游离的泛素和泛素偶联酶CrUbc13，当外源的泛素和腺苷三磷酸酶加入鞭毛时，在离体鞭毛中有几种蛋白被泛素化，提示泛素偶联系统在鞭毛中起作用。在鞭毛吸收过程中，泛素化鞭毛蛋白水平增加，特别是在鞭毛内运输（IFT）突变体中，这表明拆卸产物被泛素标记并通过IFT运输到细胞体。泛素偶联系统的底物包括α-微管蛋白（但不包括β-微管蛋白）、动力蛋白亚基（IC2）、参与交配过程的两种信号蛋白、环鸟苷单磷酸依赖激酶和阳离子通道多囊肾病2。鞭毛蛋白泛素化在交配早期增强，提示泛素化在调节鞭毛信号通路中也起积极作用。
The disassembly of cilia and flagella is linked to the cell cycle and environmental cues. We have found that ubiquitination of flagellar proteins is an integral part of flagellar disassembly. Free ubiquitin and the ubiquitin-conjugating enzyme CrUbc13 are detected in flagella, and several proteins are ubiquitinated in isolated flagella when exogenous ubiquitin and adenosine triphosphatase are added, suggesting that the ubiquitin conjugation system operates in flagella. Levels of ubiquitinated flagellar proteins increase during flagellar resorption, especially in intraflagellar transport (IFT) mutants, suggesting that disassembly products are labeled with ubiquitin and transported to the cell body by IFT. Substrates of the ubiquitin conjugation system include α-tubulin (but not β-tubulin), a dynein subunit (IC2), two signaling proteins involved in the mating process, cyclic guanosine monophosphate–dependent kinase, and the cation channel polycystic kidney disease 2. Ubiquitination of flagellar proteins is enhanced early in mating, suggesting that ubiquitination also plays an active role in regulating signaling pathways in flagella.
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影响因子：
15.9
作者：
Chauvet, V;Tian, X;Caplan, MJ
通讯作者：
Caplan, MJ
影响因子：
7.8
作者：
GOODENOUGH, UW;JURIVICH, D
通讯作者：
JURIVICH, D
Chlamydomonas Reinhardtii鞭毛中PKD2的功能和动力学。
DOI：
10.1083/jcb.200704069
发表时间：
2007-11-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Huang K;Diener DR;Mitchell A;Pazour GJ;Witman GB;Rosenbaum JL
通讯作者：
Rosenbaum JL
DOI：
10.1016/j.cub.2008.11.020
发表时间：
2008-12-23
期刊：
Current biology : CB
影响因子：
--
作者：
Lee E;Sivan-Loukianova E;Eberl DF;Kernan MJ
通讯作者：
Kernan MJ
DOI：
10.1083/jcb.141.4.993
发表时间：
1998-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cole DG;Diener DR;Himelblau AL;Beech PL;Fuster JC;Rosenbaum JL
通讯作者：
Rosenbaum JL