Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites.
Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites.
复制标题
酶联免疫吸附测定法，用于对植物次生代谢产物的定量/定性分析。
DOI：
10.1007/s11418-017-1144-z
复制
发表时间：
2018-01
影响因子：
3.3
通讯作者：
Morimoto S
中科院分区：
文献类型：
--
作者：
Sakamoto S;Putalun W;Vimolmangkang S;Phoolcharoen W;Shoyama Y;Tanaka H;Morimoto S
免疫测定是基于抗体的定量/定性分析方法。由于免疫测定的原理是基于特异性抗原-抗体反应，因此该测定已在世界范围内用于诊断、通过药物监测进行的药代动力学研究以及市售产品的质量控制。贝尔森和雅洛是第一个开发出用于检测内源性血浆胰岛素的免疫测定法（称为放射免疫测定法（RIA））的人，雅洛因此获得了1977年的诺贝尔生理学或医学奖。即使在半个世纪后的今天，免疫测定也被广泛使用，其中对最初提出的系统进行了一些修改，例如，由于与放射性使用有关的安全问题，放射性同位素已经被酶取代，这被称为酶免疫测定/酶联免疫吸附测定（ELISA）。此外，随着重组DNA技术的最新进展，ELISA也取得了进展，导致抗体、探针甚至系统的范围增加。本文综述了酶联免疫吸附法及其在植物次生代谢物检测中的应用。
Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis. Since the principle of immunoassays is based on specific antigen–antibody reaction, the assays have been utilized worldwide for diagnosis, pharmacokinetic studies by drug monitoring, and the quality control of commercially available products. Berson and Yalow were the first to develop an immunoassay, known as radioimmunoassay (RIA), for detecting endogenous plasma insulin, a development for which Yalow was awarded the Nobel Prize in Physiology or Medicine in 1977. Even today, after half a century, immunoassays are widely utilized with some modifications from the originally proposed system, e.g., radioisotopes have been replaced with enzymes because of safety concerns regarding the use of radioactivity, which is referred to as enzyme immunoassay/enzyme-linked immunosorbent assay (ELISA). In addition, progress has been made in ELISA with the recent advances in recombinant DNA technology, leading to increase in the range of antibodies, probes, and even systems. This review article describes ELISA and its applications for the detection of plant secondary metabolites.
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影响因子：
11.4
作者：
Boscolo, S.;Pelin, M.;Tubaro, A.
通讯作者：
Tubaro, A.
DOI：
10.1038/nbt0291-165
发表时间：
1991-02-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
DAVIS, GT;BEDZYK, WD;JACOBS, TW
通讯作者：
JACOBS, TW
影响因子：
5
作者：
BELANGER, L;SYLVESTRE, C;DUFOUR, D
通讯作者：
DUFOUR, D
影响因子：
2.2
作者：
COMOGLIO, S;CELADA, F
通讯作者：
CELADA, F
DOI：
10.1007/978-1-60327-254-4_3
发表时间：
2009-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
通讯作者：
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