Dung beetles as samplers of mammals in Malaysian Borneo-a test of high throughput metabarcoding of iDNA.
Dung beetles as samplers of mammals in Malaysian Borneo-a test of high throughput metabarcoding of iDNA.
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作者：
Drinkwater R;Williamson J;Clare EL;Chung AYC;Rossiter SJ;Slade E
生物多样性调查中的无脊椎动物DNA（iDNA）取样越来越普遍，大多数陆地研究依赖于从吸血无脊椎动物（如水蛭和蚊子）的肠道内容物中提取的DNA。蜣螂（金龟子总科）主要以陆生脊椎动物的粪便为食，作为脊椎动物DNA的取样器，它比吸血的无脊椎动物有几个潜在的好处。重要的是，这些甲虫可以很容易地捕获大量使用简单，廉价的诱饵陷阱，是全球性的分布，并在广泛的栖息地发生。为了建立在现有的几项研究表明，作为哺乳动物DNA的来源，蜣螂的潜力，我们进行了大型的，婆罗洲蜣螂（Catharsius renaudpauliani）的控制喂养实验。我们使用qPCR技术分析了饲喂后不同时间的肠道内容物的DNA。在这里，我们首先描述了一个粪甲虫消化道内的DNA持久性的窗口。我们发现，成功扩增牛DNA的能力在相对较短的时间内衰减，DNA拷贝数在短短6小时内减少了两个数量级。此外，我们从马来西亚沙巴的低地热带雨林中采集了蜣螂群落样本，以测试是否有可能从蜣螂iDNA中识别脊椎动物序列。我们对大型蜣螂物种的肠道内容物以及整个小型甲虫群落进行了测序。我们成功地从我们的样本中识别出六种哺乳动物物种，包括胡子猪（Sus pigatus）和水鹿（Rusa unicolor）-这两种物种都是IUCN红色名录上的脆弱物种。我们的研究结果代表了首次使用蜣螂iDNA对东南亚脊椎动物区系进行采样，并强调了蜣螂iDNA在未来生物多样性监测调查中的潜力。
Invertebrate-derived DNA (iDNA) sampling in biodiversity surveys is becoming increasingly widespread, with most terrestrial studies relying on DNA derived from the gut contents of blood-feeding invertebrates, such as leeches and mosquitoes. Dung beetles (superfamily Scarabaeoidea) primarily feed on the faecal matter of terrestrial vertebrates and offer several potential benefits over blood-feeding invertebrates as samplers of vertebrate DNA. Importantly, these beetles can be easily captured in large numbers using simple, inexpensive baited traps, are globally distributed, and occur in a wide range of habitats. To build on the few existing studies demonstrating the potential of dung beetles as sources of mammalian DNA, we subjected the large-bodied, Bornean dung beetle (Catharsius renaudpauliani) to a controlled feeding experiment. We analysed DNA from gut contents at different times after feeding using qPCR techniques. Here, we first describe the window of DNA persistence within a dung beetle digestive tract. We found that the ability to successfully amplify cattle DNA decayed over relatively short time periods, with DNA copy number decreasing by two orders of magnitude in just 6 h. In addition, we sampled communities of dung beetles from a lowland tropical rainforest in Sabah, Malaysia, in order to test whether it is possible to identify vertebrate sequences from dung beetle iDNA. We sequenced both the gut contents from large dung beetle species, as well as whole communities of smaller beetles. We successfully identified six mammalian species from our samples, including the bearded pig (Sus barbatus) and the sambar deer (Rusa unicolor)—both vulnerable species on the IUCN red list. Our results represent the first use of dung beetle iDNA to sample Southeast Asian vertebrate fauna, and highlight the potential for dung beetle iDNA to be used in future biodiversity monitoring surveys.
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影响因子：
8.8
作者：
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