Computational and Molecular Properties of Starburst Amacrine Cell Synapses Differ With Postsynaptic Cell Type.
Computational and Molecular Properties of Starburst Amacrine Cell Synapses Differ With Postsynaptic Cell Type.
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DOI：
10.3389/fncel.2021.660773
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发表时间：
2021
影响因子：
5.3
通讯作者：
Demb JB
中科院分区：
文献类型：
--
作者：
Pottackal J;Singer JH;Demb JB
突触前神经元可以通过将功能上不同的信号传输到其突触后细胞类型中的每一种来增加其计算能力。为了确定这种计算专业化是否发生在神经突乔木内的精细空间尺度上，我们研究了星爆无长突细胞（SAC）的输出突触处的计算，SAC是视网膜中经典方向选择（DS）电路的关键组成部分。SAC是一种非尖峰中间神经元，共同释放GABA和乙酰胆碱，并在两种突触后细胞类型上形成紧密间隔（<5 μm）的抑制性突触：DS神经节细胞（DSGCs）和相邻的SAC。在小鼠视网膜中的SAC的动态光遗传学刺激期间，抑制性突触后电流的全细胞记录显示，DSGCs上的GABA能突触比邻近SAC上的GABA能突触表现出更强的低通滤波。计算分析表明，这种过滤差异可以解释主要是由突触前的属性，而不是那些突触后细胞本身。与功能多样的SAC前突触一致，N型电压门控钙通道阻断可消除SAC中的GABA能电流，但仅中度降低DSGCs中的GABA能和胆碱能电流。这些结果共同证明了突触输出的专业化如何在精细的空间尺度上增强紧凑的中间神经元中的并行处理。此外，GABA能SAC突触的独特传递动力学准备支持DS电路内抑制的功能多样性。
A presynaptic neuron can increase its computational capacity by transmitting functionally distinct signals to each of its postsynaptic cell types. To determine whether such computational specialization occurs over fine spatial scales within a neurite arbor, we investigated computation at output synapses of the starburst amacrine cell (SAC), a critical component of the classical direction-selective (DS) circuit in the retina. The SAC is a non-spiking interneuron that co-releases GABA and acetylcholine and forms closely spaced (<5 μm) inhibitory synapses onto two postsynaptic cell types: DS ganglion cells (DSGCs) and neighboring SACs. During dynamic optogenetic stimulation of SACs in mouse retina, whole-cell recordings of inhibitory postsynaptic currents revealed that GABAergic synapses onto DSGCs exhibit stronger low-pass filtering than those onto neighboring SACs. Computational analyses suggest that this filtering difference can be explained primarily by presynaptic properties, rather than those of the postsynaptic cells per se. Consistent with functionally diverse SAC presynapses, blockade of N-type voltage-gated calcium channels abolished GABAergic currents in SACs but only moderately reduced GABAergic and cholinergic currents in DSGCs. These results jointly demonstrate how specialization of synaptic outputs could enhance parallel processing in a compact interneuron over fine spatial scales. Moreover, the distinct transmission kinetics of GABAergic SAC synapses are poised to support the functional diversity of inhibition within DS circuitry.
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影响因子：
8.8
作者：
Fransen, James W.;Borghuis, Bart G.
通讯作者：
Borghuis, Bart G.
影响因子：
64.8
作者：
Euler, T;Detwiler, PB;Denk, W
通讯作者：
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影响因子：
5.3
作者：
Borghuis, Bart G.;Looger, Loren L.;Demb, Jonathan B.
通讯作者：
Demb, Jonathan B.
DOI：
10.1073/pnas.1803490115
发表时间：
2018-12-18
影响因子：
11.1
作者：
Bleckert, Adam;Zhang, Chi;Wong, Rachel O.
通讯作者：
Wong, Rachel O.
影响因子：
9.8
作者：
Hausselt SE;Euler T;Detwiler PB;Denk W
通讯作者：
Denk W