Expression and characterization of rice putative PAUSED gene.
Expression and characterization of rice putative PAUSED gene.
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水稻推定 PAUSED 基因的表达和特征。
DOI：
10.1111/j.1745-7270.2008.00472.x
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发表时间：
2008-10
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
在拟南芥中，停顿(PSD)编码los1p/exportin-t的同源基因，在酵母和哺乳动物中介导转移RNA(TRNA)的核输出。然而，在水稻等单子叶植物中，对相应的直系基因的了解有限，其对生长发育和生产力的影响仍不清楚。在本研究中，我们验证了一个水稻转移DNA插入突变体PSD，并对其表型进行了分析；与野生型水稻相比，该突变体表现出严重的形态缺陷，包括发育迟缓和育性低下。Northern杂交检测野生型和突变型叶片组织中无内含子的tRNA-Tyr和含有内含子的前tRNA-Ala在细胞质和胞质中的表达水平，表明水稻PSD可能参与了tRNA从细胞核到细胞质的输出。此外，逆转录-聚合酶链式反应分析表明，PSD转录本在正常水稻植株发育过程中都有表达，亚细胞定位分析表明，水稻PSD蛋白存在于细胞核和细胞质中。综上所述，我们的研究结果表明，PSD基因可能是水稻正常发育所必需的，其功能可能与拟南芥的PSD相同。
In Arabidopsis, PAUSED (PSD) encodes the ortholog of los1p/exportin-t, which mediates the nuclear export of transfer RNA (tRNA) in yeast and mammals. However, in monocot plants such as rice, knowledge of the corresponding ortholog is limited, and its effects on growth development and productivity remain unknown. In this study, we verified a rice transfer-DNA insertional mutant psd line and analyzed its phenotypes; the mutant displayed severe morphological defects including retarded development and low fertility compared with wild-type rice. Examining intronless tRNA-Tyr and intron-containing pre-tRNA-Ala expression levels in cytoplasmic and nuclear fraction with Northern blot analysis between wild-type and mutant leaf tissue suggested that rice PSD might be involved in tRNA export from the nucleus to the cytoplasm. Additionally, reverse transcription-polymerase chain reaction analysis revealed that PSD transcript was expressed throughout normal rice plant development, and subcellular localization assays showed that rice PSD protein was present in both the nucleus and cytoplasm. In summary, our data implied that the putative PSD gene might be indispensable for normal rice development and its function might be the same as that of Arabidopsis PSD.
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影响因子：
14.9
作者：
Shibata S;Sasaki M;Miki T;Shimamoto A;Furuichi Y;Katahira J;Yoneda Y
通讯作者：
Yoneda Y
影响因子：
7.2
作者：
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通讯作者：
An, G
影响因子：
7.4
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An, SY;Park, S;An, GH
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影响因子：
6.9
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Hanson, MR;Köhler, RH
通讯作者：
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影响因子：
3.7
作者：
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C. Yao;Botao Zhao;Wei Li-;Yang Li;Wenming Qin;Bing Huang;Youxin Jin