Polyphenism in social insects: insights from a transcriptome-wide analysis of gene expression in the life stages of the key pollinator, Bombus terrestris.
Polyphenism in social insects: insights from a transcriptome-wide analysis of gene expression in the life stages of the key pollinator, Bombus terrestris.
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DOI：
10.1186/1471-2164-12-623
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发表时间：
2011-12-20
期刊：
影响因子：
4.4
通讯作者：
Brown MJ
中科院分区：
文献类型：
--
作者：
Colgan TJ;Carolan JC;Bridgett SJ;Sumner S;Blaxter ML;Brown MJ
了解多态性，一个单一的基因组表达多种形态和行为不同的表型的能力，是进化和发育生物学的一个重要目标。多型性一直是水螅类进化的关键，特别是社会水螅类，其中一个物种的基因组调节不同的幼虫阶段，两性异形和雌性内的物理种姓。因此，对社会Hyplitera的转录组学分析将为基因表达的变化如何构成这种复杂性提供独特的见解。在这里，我们描述了基因表达的个体标本的成年前阶段，性别和种姓的关键传粉者，浅黄色尾熊蜂terrestris。从五个生命周期阶段（一个幼虫，一个蛹，一个雄性，一个雌核和两个工人）的mRNA制备cDNA，并使用Roche 454技术生成了总共1，610，742个表达序列标签（EST），大大增加了这个重要物种的序列数据。重叠的EST被组装成36，354个B。terrestris推定的转录本，并功能注释。使用R-STAT分析对来自成体前阶段、种姓和性别的非重复样本的基因表达差异进行初步评估。来自大黄蜂生命周期各阶段的个体样本在一系列基因的表达上存在差异，包括涉及氨基酸储存、代谢、免疫和嗅觉的基因。对免疫和嗅觉基因在不同表型中表达的详细分析证明了转录组学分析如何为我们理解B生物学的核心过程提供信息。terrestris和一般的社会Hypletera。例如，免疫相关基因的检查确定了生命周期阶段个体标本中重要免疫途径组分的高度保守性，而嗅觉相关基因表现出差异表达，与未成熟阶段相比，成年人，特别是性行为者的基因表达谱更宽。由于在样品中没有复制，本研究的结果是初步的，但提供了许多可能与不同表型阶段表达相关的候选基因。这个全面的转录组目录将提供一个重要的基因发现资源，在生态学，进化和保护的一个关键传粉者定向方案。
Understanding polyphenism, the ability of a single genome to express multiple morphologically and behaviourally distinct phenotypes, is an important goal for evolutionary and developmental biology. Polyphenism has been key to the evolution of the Hymenoptera, and particularly the social Hymenoptera where the genome of a single species regulates distinct larval stages, sexual dimorphism and physical castes within the female sex. Transcriptomic analyses of social Hymenoptera will therefore provide unique insights into how changes in gene expression underlie such complexity. Here we describe gene expression in individual specimens of the pre-adult stages, sexes and castes of the key pollinator, the buff-tailed bumblebee Bombus terrestris. cDNA was prepared from mRNA from five life cycle stages (one larva, one pupa, one male, one gyne and two workers) and a total of 1,610,742 expressed sequence tags (ESTs) were generated using Roche 454 technology, substantially increasing the sequence data available for this important species. Overlapping ESTs were assembled into 36,354 B. terrestris putative transcripts, and functionally annotated. A preliminary assessment of differences in gene expression across non-replicated specimens from the pre-adult stages, castes and sexes was performed using R-STAT analysis. Individual samples from the life cycle stages of the bumblebee differed in the expression of a wide array of genes, including genes involved in amino acid storage, metabolism, immunity and olfaction. Detailed analyses of immune and olfaction gene expression across phenotypes demonstrated how transcriptomic analyses can inform our understanding of processes central to the biology of B. terrestris and the social Hymenoptera in general. For example, examination of immunity-related genes identified high conservation of important immunity pathway components across individual specimens from the life cycle stages while olfactory-related genes exhibited differential expression with a wider repertoire of gene expression within adults, especially sexuals, in comparison to immature stages. As there is an absence of replication across the samples, the results of this study are preliminary but provide a number of candidate genes which may be related to distinct phenotypic stage expression. This comprehensive transcriptome catalogue will provide an important gene discovery resource for directed programmes in ecology, evolution and conservation of a key pollinator.
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DOI：
10.1016/j.cbpb.2008.02.007
发表时间：
2008-06-01
影响因子：
2.2
作者：
Choi, Yong Soo;Choo, Young Moo;Jin, Byung Rae
通讯作者：
Jin, Byung Rae
影响因子：
3.8
作者：
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通讯作者：
Maleszka, Ryszard
影响因子：
3.7
作者：
Chen S;Yao H;Han J;Liu C;Song J;Shi L;Zhu Y;Ma X;Gao T;Pang X;Luo K;Li Y;Li X;Jia X;Lin Y;Leon C
通讯作者：
Leon C
影响因子：
1.7
作者：
CAMERON, SA
通讯作者：
CAMERON, SA
DOI：
10.1073/pnas.96.10.5575
发表时间：
1999-05-11
影响因子：
11.1
作者：
Evans, JD;Wheeler, DE
通讯作者：
Wheeler, DE