Gene transcript abundance profiles distinguish Kawasaki disease from adenovirus infection.
Gene transcript abundance profiles distinguish Kawasaki disease from adenovirus infection.
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DOI：
10.1086/603538
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发表时间：
2009-08-15
期刊：
影响因子：
--
通讯作者：
Relman DA
中科院分区：
文献类型：
--
作者：
Popper SJ;Watson VE;Shimizu C;Kanegaye JT;Burns JC;Relman DA
急性川崎病（KD）很难与其他涉及急性皮疹或发热的疾病区分开来，部分原因是病原体和病理生理学特征仍然很差。因此，诊断和关键治疗可能会延迟。我们使用DNA微阵列来鉴定KD的可能诊断特征。我们比较了23名急性KD患儿和18名年龄匹配的患有3种类似KD疾病的发热儿童血液中的基因表达模式。与血小板和中性粒细胞活化相关的基因在KD患者中的表达水平高于急性腺病毒感染或全身药物不良反应患者，但KD患者的表达水平并不高于猩红热患者。与B细胞活化相关的基因在KD患者中的表达水平也高于对照组。在一个独立的KD患者队列中证实了KD患者中干扰素刺激的基因表达显著缺失。使用一组38个基因转录本，我们成功地预测了23例KD患者中的21例和8例腺病毒感染患者中的7例的诊断。这些发现提供了深入了解KD与其他发热性疾病的分子特征，并支持基于宿主反应开发KD新型诊断试剂的可行性。
Acute Kawasaki disease (KD) is difficult to distinguish from other illnesses that involve acute rash or fever, in part because the etiologic agent(s) and pathophysiology remain poorly characterized. As a result, diagnosis and critical therapies may be delayed. We used DNA microarrays to identify possible diagnostic features of KD. We compared gene expression patterns in the blood of 23 children with acute KD and 18 age-matched febrile children with 3 illnesses that resemble KD. Genes associated with platelet and neutrophil activation were expressed at higher levels in patients with KD than in patients with acute adenovirus infections or systemic adverse drug reactions, but levels in patients with KD were not higher than those in patients with scarlet fever. Genes associated with B cell activation were also expressed at higher levels in patients with KD than in control subjects. A striking absence of interferon-stimulated gene expression in patients with KD was confirmed in an independent cohort of patients with KD. Using a set of 38 gene transcripts, we successfully predicted the diagnosis for 21 of 23 patients with KD and 7 of 8 patients with adenovirus infection. These findings provide insight into the molecular features that distinguish KD from other febrile illnesses and support the feasibility of developing novel diagnostic reagents for KD based on the host response.
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14.9
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DOI：
10.1016/0090-1229(82)90075-7
发表时间：
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期刊：
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY
影响因子：
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作者：
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影响因子：
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作者：
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