Transient transcriptional silencing alters the cell cycle to promote germline stem cell differentiation in Drosophila.
Transient transcriptional silencing alters the cell cycle to promote germline stem cell differentiation in Drosophila.
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DOI：
10.1016/j.ydbio.2017.11.014
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发表时间：
2018-02-01
影响因子：
2.7
通讯作者：
Rangan P
中科院分区：
文献类型：
--
作者：
Flora P;Schowalter S;Wong-Deyrup S;DeGennaro M;Nasrallah MA;Rangan P
转录沉默是胚胎生殖细胞用来抑制体细胞命运和维持全能性和永生的保守过程。在果蝇中，这种转录沉默是由极性颗粒成分（pgc）介导的。在这里，我们表明，在成年卵巢，PGC是所需的及时生殖干细胞（GSC）分化。Pgc在直接GSC子代（前成囊细胞）中瞬时表达，在那里它介导转录沉默脉冲。当分化因子bag of marbles（bam）表达时，由pgc介导的这种转录沉默间接促进细胞周期蛋白B（CycB）的积累和细胞周期进展到晚G2期。Pgc介导的CycB积累也是异染色质沉积所必需的，其保护生殖系基因组免受自私DNA元件的影响。我们的研究结果表明，在前成囊细胞的瞬时转录沉默“重新编程”，它远离自我更新和对配子分化程序。
Transcriptional silencing is a conserved process used by embryonic germ cells to repress somatic fate and maintain totipotency and immortality. In Drosophila, this transcriptional silencing is mediated by polar granule component (pgc). Here, we show that in the adult ovary, pgc is required for timely germline stem cell (GSC) differentiation. Pgc is expressed transiently in the immediate GSC daughter (pre-cystoblast), where it mediates a pulse of transcriptional silencing. This transcriptional silencing mediated by pgc indirectly promotes the accumulation of Cyclin B (CycB) and cell cycle progression into late-G2 phase, when the differentiation factor bag of marbles (bam) is expressed. Pgc mediated accumulation of CycB is also required for heterochromatin deposition, which protects the germ line genome against selfish DNA elements. Our results suggest that transient transcriptional silencing in the pre-cystoblast “re-programs” it away from self-renewal and toward the gamete differentiation program.
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影响因子：
10.5
作者：
MCKEARIN, DM;SPRADLING, AC
通讯作者：
SPRADLING, AC
影响因子：
11.8
作者：
Mathieu J;Cauvin C;Moch C;Radford SJ;Sampaio P;Perdigoto CN;Schweisguth F;Bardin AJ;Sunkel CE;McKim K;Echard A;Huynh JR
通讯作者：
Huynh JR
影响因子：
64.5
作者：
Mohn, Fabio;Sienski, Grzegorz;Brennecke, Julius
通讯作者：
Brennecke, Julius
DOI：
10.1523/jneurosci.1862-10.2010
发表时间：
2010-11-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Astigarraga S;Hofmeyer K;Farajian R;Treisman JE
通讯作者：
Treisman JE
影响因子：
23.9
作者：
Jin, Zhigang;Kirilly, Daniel;Xie, Ting
通讯作者：
Xie, Ting