Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR.
Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR.
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低样本量基于折纸纸的石墨烯修饰适体传感器用于癌症生物标志物-EGFR的无标记电化学检测
DOI：
10.1038/s41378-020-0146-2
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发表时间：
2020
影响因子：
7.9
通讯作者：
Cai X
中科院分区：
文献类型：
--
作者：
Wang Y;Sun S;Luo J;Xiong Y;Ming T;Liu J;Ma Y;Yan S;Yang Y;Yang Z;Reboud J;Yin H;Cooper JM;Cai X
本工作采用抗表皮生长因子受体（EGFR）适体作为生物识别元件，制备了一种电化学纸基适体传感器，用于无标记、超灵敏地检测表皮生长因子受体（EGFR）。该装置采用折纸的概念，作为样品引入和检测之间的阀门，从而减少了采样体积并提高了操作便利性。氨基官能化的石墨烯（NH 2-GO）/噻吩（THI）/金粒子（AuNP）纳米复合材料用于修饰工作电极，不仅产生电化学信号，而且提供有利于适体固定的环境。电化学表征表明，绝缘适体-抗原免疫复合物的形成将阻碍电子从样品介质转移到工作电极，从而导致较低的信号。实验结果表明，该适体传感器对EGFR的线性范围为0.05 ~ 200 ngmL−1（R2= 0.989），检测限为5 pgmL− 1。通过分析血清样本进一步研究了所提出的纸质适体传感器的分析可靠性，结果与金标准酶联免疫吸附试验具有良好的一致性。
In this work, an electrochemical paper-based aptasensor was fabricated for label-free and ultrasensitive detection of epidermal growth factor receptor (EGFR) by employing anti-EGFR aptamers as the bio-recognition element. The device used the concept of paper-folding, or origami, to serve as a valve between sample introduction and detection, so reducing sampling volumes and improving operation convenience. Amino-functionalized graphene (NH2-GO)/thionine (THI)/gold particle (AuNP) nanocomposites were used to modify the working electrode not only to generate the electrochemical signals, but also to provide an environment conducive to aptamer immobilization. Electrochemical characterization revealed that the formation of an insulating aptamer–antigen immunocomplex would hinder electron transfer from the sample medium to the working electrode, thus resulting in a lower signal. The experimental results showed that the proposed aptasensor exhibited a linear range from 0.05 to 200 ngmL−1(R2= 0.989) and a detection limit of 5 pgmL−1for EGFR. The analytical reliability of the proposed paper-based aptasensor was further investigated by analyzing serum samples, showing good agreement with the gold-standard enzyme-linked immunosorbent assay.
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影响因子：
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作者：
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通讯作者：
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通讯作者：
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