Electrical detection of cancer biomarker using aptamers with nanogap break-junctions.
Electrical detection of cancer biomarker using aptamers with nanogap break-junctions.
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DOI：
10.1088/0957-4484/23/27/275502
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发表时间：
2012-07-11
期刊：
影响因子：
3.5
通讯作者：
Iqbal SM
中科院分区：
文献类型：
--
作者：
Ilyas A;Asghar W;Allen PB;Duhon H;Ellington AD;Iqbal SM
表皮生长因子受体（EGFR）是在癌细胞中过表达的细胞表面蛋白。它是已知的最常见的致癌物。EGFR浓度在癌症患者的血清中也增加。检测EGFR浓度的微小变化对于早期诊断至关重要，从而更好地治疗和提高癌症患者的生存率。本文报道了一种基于RNA适体的选择性捕获EGFR蛋白的方法及其检测的电子方案。采用聚焦离子束刻蚀和电迁移相结合的方法制备了纳米间距的金电极对。适体与单链DNA分子杂交，单链DNA分子又固定在金纳米电极之间的SiO2表面上。通过使用具有突变的非选择性适体和不具有适体的对照芯片来证明适体的选择性。通过光学检测来表征表面功能化，并且当选择性捕获EGFR时，测量到直流电（DC）增加两个数量级。这代表了用于检测医学应用中感兴趣的蛋白质的电子生物传感器。
Epidermal Growth Factor Receptor (EGFR) is a cell surface protein overexpressed in cancerous cells. It is known to be the most common oncongene. EGFR concentration also increases in the serum of cancer patients. The detection of small changes in the concentration of EGFR can be critical for early diagnosis, resulting in better treatment and improved survival rate of cancer patients. This article reports an RNA aptamer based approach to selectively capture EGFR protein and an electrical scheme for its detection. Pairs of gold electrodes with nanometer separation were made through confluence of focused ion beam scratching and electromigration. The aptamer was hybridized to a single stranded DNA molecule, which in turn was immobilized on SiO2 surface between the gold nanoelectrodes. The selectivity of the aptamer was demonstrated by using control chips with mutated non–selective aptamer and with no aptamer. Surface functionalization was characterized by optical detection and two orders of magnitude increase in direct current (DC) was measured when selective capture of EGFR occurred. This represents an electronic biosensor for the detection of proteins of interest for medical applications.
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DOI：
10.1007/bf02482343
发表时间：
1999-01-01
期刊：
SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY
影响因子：
--
作者：
Inada, S;Koto, T;Iwashita, A
通讯作者：
Iwashita, A
DOI：
10.1115/1.4001664
发表时间：
2010-06-01
影响因子：
4
作者：
Asghar, Waseem;Ramachandran, Priyanka P.;Iqbal, Samir M.
通讯作者：
Iqbal, Samir M.
影响因子：
8.6
作者：
Datta, S;Tian, WD;Kubiak, CP
通讯作者：
Kubiak, CP
影响因子：
4
作者：
Liu, K;Avouris, P;Michl, J
通讯作者：
Michl, J
影响因子：
2.9
作者：
Hamaguchi, N;Ellington, A;Stanton, M
通讯作者：
Stanton, M