Heat inactivation of the Middle East respiratory syndrome coronavirus.

Heat inactivation of the Middle East respiratory syndrome coronavirus.
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DOI:
10.1111/irv.12261
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发表时间:
2014-09
影响因子:
4.4
通讯作者:
Manuguerra JC
Manuguerra JC
中科院分区:
医学4区
文献类型:
--
作者:
Leclercq I;Batéjat C;Burguière AM;Manuguerra JC

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将新出现的中东呼吸综合征冠状病毒 (MERS-CoV) 的培养上清液随时间置于三个温度下,并通过 TCID50 方法在 Vero E6 细胞上测试感染性。在 56°C 下,需要大约 25 分钟才能将初始滴度降低 4 log10。将温度升高至 65°C 对病毒感染性产生强烈的负面影响,因为病毒杀伤力显着下降至 1 分钟。相反,在25℃下2小时后没有观察到效价的显着下降。这些数据可能有助于在实验室中制定针对中东呼吸综合征冠状病毒的生物安全措施。
The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log10. Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.
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