Cyclic di-GMP regulates bacterial colonization and further biocontrol efficacy of Bacillus velezensis against apple ring rot disease via its potential receptor YdaK.
Cyclic di-GMP regulates bacterial colonization and further biocontrol efficacy of Bacillus velezensis against apple ring rot disease via its potential receptor YdaK.
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环状双GMP通过其潜在的受体YdaK调节细菌定殖，进而调节绒毛芽孢杆菌对苹果轮纹病的生防效果。
DOI：
10.3389/fmicb.2022.1034168
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发表时间：
2022
影响因子：
5.2
通讯作者：
Li, Yan
中科院分区：
文献类型：
--
作者：
Gong, Huiling;Jiang, Wenxiao;Yang, Yang;Zhang, Yue;Chen, Xufei;Li, Wei;Yang, Panlei;Wang, Zhenshuo;Wang, Qi;Li, Yan
芽孢杆菌属是研究最多的有益细菌之一，广泛用作农业系统中的生物防治剂。其生物防治功效由多种调节剂控制。环状二鸟苷酸 (c-di-GMP) 是细菌中几乎普遍存在的第二信使，可调节各种重要的生理过程，包括运动、生物膜形成、抗真菌抗生素产生和宿主定植。然而，c-di-GMP 对有益菌生防效果的影响尚不清楚。 Bacillus velezensis PG12 是一种针对 Botryosphaeria dothidea 引起的苹果环腐病的有效生防菌株。在本研究中，研究了 c-di-GMP 对 B. velezensis PG12 生物防治功效的贡献。编码二鸟苷酸环化酶或磷酸二酯酶的单个基因的缺失并不影响其对苹果环腐病的生防效果。然而，人为调节细胞中的c-di-GMP水平会导致生防效力发生显着变化，表明c-di-GMP正向调节B. velezensis PG12对苹果环腐病的生防效力。更多证据表明，c-di-GMP在体外和体内均不影响B. velezensis PG12对B. dothidea的拮抗活性，但正向调节B. velezensis PG12生物膜的形成及其在苹果果实上的定植。重要的是，ydaK的缺失可以挽救低c-di-GMP水平引起的生物膜形成、细菌定植和生物防治效果的抑制，表明YdaK是调节生物膜形成、定植和有效生物防治的潜在c-di-GMP受体。然而，YdaK不影响B. velezensis PG12对B. dothidea的拮抗活性。基于这些发现，我们提出 c-di-GMP 调节生物膜的形成，随后调节苹果果实上的细菌定植，从而通过其受体 YdaK 调节贝莱森巴氏杆菌的生物防治功效。这是第一份表明 c-di-GMP 在有益细菌的生物防治功效中发挥作用的报告。
Bacillus species are among the most investigated beneficial bacteria and widely used in agricultural systems as biological control agents. Its biocontrol efficacy is controlled by diverse regulators. Cyclic diguanylate (c-di-GMP) is a nearly universal second messenger in bacteria and modulates various important physiological processes, including motility, biofilm formation, antifungal antibiotic production and host colonization. However, the impact of c-di-GMP on biocontrol efficacy of beneficial bacteria is unknown. Bacillus velezensis PG12 is an effective biocontrol strain against apple ring rot disease caused by Botryosphaeria dothidea. In this study, the contribution of c-di-GMP to biocontrol efficacy of B. velezensis PG12 was investigated. Deletion of single gene encoding diguanylate cyclase or phosphodiesterase did not affect its biocontrol efficacy against apple ring rot. However, artificial modulation of c-di-GMP level in the cells leads to a significant change of biocontrol efficacy, suggesting that c-di-GMP positively regulates biocontrol efficacy of B. velezensis PG12 against apple ring rot disease. More evidences indicate that c-di-GMP does not affect the antagonistic activity of B. velezensis PG12 against B. dothidea in vitro and in vivo, but positively regulates biofilm formation of B. velezensis PG12 and its colonization on apple fruits. Importantly, deletion of ydaK could rescue the inhibition of biofilm formation, bacterial colonization and biocontrol efficacy caused by low c-di-GMP level, indicating that YdaK is the potential c-di-GMP receptor to regulate biofilm formation, colonization and effective biological control. However, YdaK did not affect the antagonistic activity of B. velezensis PG12 against B. dothidea. Based on these findings, we propose that c-di-GMP regulates biofilm formation, subsequently the bacterial colonization on apple fruits and thus biocontrol efficacy of B. velezensis through its receptor YdaK. This is the first report showing that c-di-GMP plays a role in biocontrol efficacy of beneficial bacteria.
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影响因子：
4.5
作者：
Fernández-Llamosas H;Díaz E;Carmona M
通讯作者：
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影响因子：
4.1
作者：
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影响因子：
3.3
作者：
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影响因子：
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作者：
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影响因子：
2.7
作者：
Chen, Xue-Ting;Ji, Jun-Bin;Yan, Xin
通讯作者：
Yan, Xin