L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation.
L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation.
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DOI：
10.3389/fcimb.2021.698146
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发表时间：
2021
影响因子：
5.7
通讯作者：
Gunn JS
中科院分区：
文献类型：
--
作者：
Vasicek EM;O'Neal L;Parsek MR;Fitch J;White P;Gunn JS
L-阿拉伯糖诱导型启动子常用于基因表达分析。然而，营养来源和可用性也在生物膜形成中发挥作用；因此，L-阿拉伯糖代谢可能会影响生物膜的发育。在这项研究中，我们研究了 L-阿拉伯糖对鼠伤寒沙门氏菌（S. Typhimurium）生物膜形成的影响。使用 L-阿拉伯糖转运和代谢受损的突变体，我们发现 L-阿拉伯糖代谢对体外鼠伤寒沙门氏菌生物膜形成产生负面影响。当 L-阿拉伯糖代谢被消除时，生物膜形成恢复到基线水平。然而，如果没有导入细胞外 L-阿拉伯糖的能力，生物膜的形成就会显着增加。使用 RNA-Seq，我们鉴定了参与这些不同表型的几个基因家族，包括 curli 表达、氨基酸合成和 L-阿拉伯糖代谢。测试了几个单独的候选基因是否参与 L-阿拉伯糖介导的生物膜表型，但大多数没有发挥重要作用。有趣的是，在存在 L-阿拉伯糖的情况下，野生型鼠伤寒沙门氏菌中的二鸟苷酸环化酶基因 adrA 下调。同时，编码腺苷酸环化酶的 cyaA 在 L-阿拉伯糖转运突变体中下调。使用 IPTG 诱导质粒通过 vieA 表达耗尽 c-di-GMP，我们能够消除在转运突变体中看到的增加的生物膜表型。然而，L-阿拉伯糖输入突变体形成明显更大的生物膜的机制仍有待确定。无论如何，这些数据表明 L-阿拉伯糖代谢影响细胞内 c-di-GMP 水平，从而影响生物膜的形成。当考虑在生物膜条件下使用 L-阿拉伯糖诱导型启动子时，这些发现很重要。
L-arabinose inducible promoters are commonly used in gene expression analysis. However, nutrient source and availability also play a role in biofilm formation; therefore, L-arabinose metabolism could impact biofilm development. In this study we examined the impact of L-arabinose on Salmonella enterica serovar Typhimurium (S. Typhimurium) biofilm formation. Using mutants impaired for the transport and metabolism of L-arabinose, we showed that L-arabinose metabolism negatively impacts S. Typhimurium biofilm formation in vitro. When L-arabinose metabolism is abrogated, biofilm formation returned to baseline levels. However, without the ability to import extracellular L-arabinose, biofilm formation significantly increased. Using RNA-Seq we identified several gene families involved in these different phenotypes including curli expression, amino acid synthesis, and L-arabinose metabolism. Several individual candidate genes were tested for their involvement in the L-arabinose-mediated biofilm phenotypes, but most played no significant role. Interestingly, in the presence of L-arabinose the diguanylate cyclase gene adrA was downregulated in wild type S. Typhimurium. Meanwhile cyaA, encoding an adenylate cyclase, was downregulated in an L-arabinose transport mutant. Using an IPTG-inducible plasmid to deplete c-di-GMP via vieA expression, we were able to abolish the increased biofilm phenotype seen in the transport mutant. However, the mechanism by which the L-arabinose import mutant forms significantly larger biofilms remains to be determined. Regardless, these data suggest that L-arabinose metabolism influences intracellular c-di-GMP levels and therefore biofilm formation. These findings are important when considering the use of an L-arabinose inducible promoter in biofilm conditions.
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影响因子：
10.5
作者：
Dahlstrom KM;O'Toole GA
通讯作者：
O'Toole GA
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.2
作者：
GUTNICK, D;CALVO, JM;AMES, BN
通讯作者：
AMES, BN
影响因子：
3.2
作者：
BROWN, CE;HOGG, RW
通讯作者：
HOGG, RW
影响因子：
16.6
作者：
Chua, Song Lin;Liu, Yang;Yang, Liang
通讯作者：
Yang, Liang