Continuous monitoring of changes in plasma nitrite following cerebral ischemia in a rabbit embolic stroke model.
Continuous monitoring of changes in plasma nitrite following cerebral ischemia in a rabbit embolic stroke model.
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DOI：
10.1007/s12975-011-0073-5
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发表时间：
2011-06
影响因子：
6.9
通讯作者：
Lapchak, Paul A.
中科院分区：
文献类型：
--
作者：
Silver, James H.;Lapchak, Paul A.
在这项概念验证研究中，我们研究了通过留置颈动脉导管直接、连续监测血浆亚硝酸盐作为兔血栓栓塞术后脑缺血的指标。比较两组兔动脉栓塞术对亚硝酸盐水平的影响。对照组采用颈静脉置管持续采血。血液立即通过超滤过滤器；滤液经过化学还原，将游离亚硝酸盐转化为一氧化氮(NO)，然后使用NO专用电极进行测量。栓塞组在达到基线亚硝酸盐水平后，经颈动脉导管将血块注入脑内，然后从颈静脉血中连续测定亚硝酸盐水平。与对照组相比，中风组的亚硝酸盐显著增加(p=0.017)。采用曲线下面积(AUC)法，在栓塞后3min内，结果达到统计学意义(p<；0.05)。栓塞组NO2水平较术前升高424±256%。这项研究表明，亚硝酸盐可以在中风后立即测量，并且我们的系统测量亚硝酸盐与中风的程度无关。本研究为使用亚硝酸盐作为缺血性卒中标志物的可行性提供了证据。
In this proof-of-concept study, we investigated direct, continuous monitoring of plasma nitrite as an indicator of cerebral ischemia following clot embolization of rabbits via an indwelling carotid catheter. Two groups of rabbits were studied to compare the effects of embolization on nitrite levels. In the control group, blood was continuously obtained from a jugular venous catheter. The blood was immediately passed through an ultrafiltration filter; the filtrate was chemically reduced to convert free nitrite to nitric oxide (NO) and then measured using a NO-specific electrode. In the embolized group, after a baseline nitrite level was achieved, blood clots were injected into the brain via the carotid artery catheter, and then nitrite levels were continuously measured from jugular venous blood. The stroke group showed a significantly greater increase in nitrite as compared to controls (p=0.017). Using the area-under-the-curve (AUC) method, results reached statistical significance (p<0.05) within 3 min of embolization. In embolized rabbits, NO2 levels increased 424±256% compared to baseline. This study shows that nitrite can be measured immediately following a stroke and that our system measures nitrite independent of the extent of the stroke. This study provides evidence for the feasibility of using nitrite as a marker of ischemic stroke.
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DOI：
10.1186/cc7706
发表时间：
2009
期刊：
Critical care (London, England)
影响因子：
--
作者：
Hollenberg SM;Cinel I
通讯作者：
Cinel I
影响因子：
5.7
作者：
Murkin, John M.;Adams, Sandra J.;Fox, Stephanie
通讯作者：
Fox, Stephanie
影响因子：
37.8
作者：
Lloyd-Jones, Donald;Adams, Robert;Hong, Yuling
通讯作者：
Hong, Yuling
影响因子：
5.5
作者：
Seals, Douglas R.;Walker, Ashley E.;Lesniewski, Lisa A.
通讯作者：
Lesniewski, Lisa A.
影响因子：
20.3
作者：
Dejam, A;Hunter, CJ;Schechter, AN
通讯作者：
Schechter, AN