Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle.
Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle.
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DOI：
10.1038/s41467-021-24646-z
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发表时间：
2021-07-27
影响因子：
16.6
通讯作者：
Lidbury IDEA
中科院分区：
文献类型：
--
作者：
Murphy ARJ;Scanlan DJ;Chen Y;Adams NBP;Cadman WA;Bottrill A;Bending G;Hammond JP;Hitchcock A;Wellington EMH;Lidbury IDEA
还原有机磷分子，如烷基膦酸酯和氨基膦酸酯的质子交换合成，代表了巨大的全球海洋磷氧化还原循环的一半。虽然烷基膦酸盐倾向于在难溶的溶解有机物中积累，但氨基膦酸盐不会。在这里，我们确定了三个细菌2-氨基乙基膦酸盐（2AEP）转运蛋白，AepXVW，AepP和AepSTU，其合成是独立的磷酸盐浓度（磷酸盐不敏感）。AepXVW存在于多种海洋异养生物中，广泛分布于中层和上层沃茨。与原型膦酸盐结合蛋白PhnD不同，AepX对2AEP具有高亲和力和高特异性（Stappia stellulata AepX Kd 23 ± 4 nM;甲基膦酸盐Kd 3.4 ± 0.3 mM）。在全球海洋中，aepX被大量转录（~100倍>phnD），与磷酸盐和氮浓度无关。总的来说，我们的数据确定了一种机制，负责海洋磷氧化还原循环中的主要氧化过程，并建议2AEP可能是再生磷酸盐和铵，这是海洋初级生产所需的一个重要来源。在这里，作者表明，2-氨基乙基膦酸盐（2AEP）矿化在全球海洋中广泛存在，独立于外源无机磷酸盐浓度。他们建议2AEP可能是支持海洋初级生产所需的磷酸盐再生的主要途径。
The planktonic synthesis of reduced organophosphorus molecules, such as alkylphosphonates and aminophosphonates, represents one half of a vast global oceanic phosphorus redox cycle. Whilst alkylphosphonates tend to accumulate in recalcitrant dissolved organic matter, aminophosphonates do not. Here, we identify three bacterial 2-aminoethylphosphonate (2AEP) transporters, named AepXVW, AepP and AepSTU, whose synthesis is independent of phosphate concentrations (phosphate-insensitive). AepXVW is found in diverse marine heterotrophs and is ubiquitously distributed in mesopelagic and epipelagic waters. Unlike the archetypal phosphonate binding protein, PhnD, AepX has high affinity and high specificity for 2AEP (Stappia stellulata AepX Kd 23 ± 4 nM; methylphosphonate Kd 3.4 ± 0.3 mM). In the global ocean, aepX is heavily transcribed (~100-fold>phnD) independently of phosphate and nitrogen concentrations. Collectively, our data identifies a mechanism responsible for a major oxidation process in the marine phosphorus redox cycle and suggests 2AEP may be an important source of regenerated phosphate and ammonium, which are required for oceanic primary production. Here the authors show that 2-aminoethylphosphonate (2AEP) mineralisation is widespread in the global ocean, operating independently of exogenous inorganic phosphate concentration. They propose 2AEP may be a major route for the regeneration of phosphate required to support marine primary production.
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影响因子：
5.6
作者：
Alicea I;Marvin JS;Miklos AE;Ellington AD;Looger LL;Schreiter ER
通讯作者：
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影响因子：
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作者：
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作者：
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作者：
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Metcalf, William W.
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作者：
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通讯作者：
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