Evolutionarily conserved bacterial effectors hijack abscisic acid signaling to induce an aqueous environment in the apoplast.
Evolutionarily conserved bacterial effectors hijack abscisic acid signaling to induce an aqueous environment in the apoplast.
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DOI：
10.1016/j.chom.2022.02.006
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发表时间：
2022-04-13
影响因子：
30.3
通讯作者：
Moffett, Peter
中科院分区：
文献类型：
--
作者：
Roussin-Leveillee, Charles;Lajeunesse, Gaele;St-Amand, Meliane;Veerapen, Varusha Pillay;Silva-Martins, Guilherme;Nomura, Kinya;Brassard, Sandrine;Bolaji, Ayooluwa;He, Sheng Yang;Moffett, Peter
较高的大气湿度水平能够建立有利于病原体的水生生存空间，从而深刻地影响植物中寄主与病原体的相互作用。细菌病原菌紫丁香假单胞菌的效应子HopM1和AvrE1已被证明在这种条件下诱导水中的质外体。然而，这种情况发生的机制仍不清楚。在这里，我们证明了HopM1和AvrE1通过诱导拟南芥转录组景观的重大重新编程来建立一个含水的生活空间。这些效应物诱导强烈的脱落酸(ABA)信号，促进气孔关闭，导致叶片蒸腾和浸水损伤减少。此外，这些效应器优先增加控制气孔开度的保卫细胞中ABA的积累。值得注意的是，一个保卫细胞特异性的ABA转运蛋白ABCG40对于HopM1诱导水浸泡损伤是必要的。这项研究为气孔操纵的一系列事件提供了分子洞察力，这些事件创造了促进感染的理想微环境。Roussin-Léveillée等。证明了两个紫丁香假单胞菌效应蛋白诱导拟南芥中脱落酸(ABA)的生物合成和信号转导。虽然在感染早期气孔开放允许进入叶质外体，但它们表明ABA诱导气孔在后期关闭，导致有利于细菌的水浸泡损伤。
High atmospheric humidity levels profoundly impact host-pathogen interactions in plants by enabling the establishment of an aqueous living space that benefits pathogens. The effectors HopM1 and AvrE1 of the bacterial pathogen Pseudomonas syringae have been shown to induce an aqueous apoplast under such conditions. However, the mechanisms by which this happens remain unknown. Here, we show that HopM1 and AvrE1 work redundantly to establish an aqueous living space by inducing a major reprogramming of the Arabidopsis thaliana transcriptome landscape. These effectors induce a strong abscisic acid (ABA) signature, which promotes stomatal closure, resulting in reduced leaf transpiration, and water-soaking lesions. Furthermore, these effectors preferentially increase ABA accumulation in guard cells, which control stomatal aperture. Notably, a guard-cell specific ABA transporter, ABCG40, is necessary for HopM1 induction of water-soaking lesions. This study provides molecular insights into a chain of events of stomatal manipulation that create an ideal microenvironment to facilitate infection. Roussin-Léveillée et al. demonstrate that two Pseudomonas syringae effector proteins induce abscisic acid (ABA) biosynthesis and signaling in Arabidopsis. Although stomatal opening early in infection allows access to the leaf apoplast, they show that ABA induces stomatal closure in later stages, leading to water soaking lesions that benefit the bacteria.
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