Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications.

Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications.
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DOI:
10.1016/j.xpro.2021.100422
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发表时间:
2021-06-18
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影响因子:
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通讯作者:
Longhi MP
Longhi MP
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其他
文献类型:
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作者:
Hearnden R;Sandhar B;Vyas V;Longhi MP

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This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to. Isolation of viable stromal vascular fraction cells from adipose tissue Applicable to multiple adipose tissue depots from human and mouse Highly reproducible adipose tissue digestion for various downstream assays This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations.
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