Use of panitumumab-IRDye800 to image microscopic head and neck cancer in an orthotopic surgical model.
Use of panitumumab-IRDye800 to image microscopic head and neck cancer in an orthotopic surgical model.
复制标题
DOI：
10.1245/s10434-012-2435-y
复制
发表时间：
2012-11
影响因子：
3.7
通讯作者：
Rosenthal EL
中科院分区：
文献类型：
--
作者：
Heath CH;Deep NL;Sweeny L;Zinn KR;Rosenthal EL
Fluorescence imaging hardware (SPY) has recently been developed for intraoperative assessment of blood flow via detection of probes emitting in the near-infrared (NIR) spectrum. This study sought to determine if this imaging system was capable of detecting micrometastatic head and neck squamous cell carcinoma (HNSCC) in preclinical models. A NIR fluorescent probe (IRDye800CW) was covalently linked to a monoclonal antibody targeting EGFR (panitumumab) or non-specific IgG. HNSCC flank (SCC-1) and orthotopic (FADU and OSC19) xenografts were imaged 48-96hrs following systemic injection of labeled panitumumab or IgG. The primary tumor and regional lymph nodes were dissected using fluorescence guidance with the SPY system and grossly assessed with a charge-coupled NIR system (Pearl). Histologic slides were also imaged with a NIR charged-coupled device (Odyssey) and fluorescence intensity was correlated with pathologic confirmation of disease. Orthotopic tongue tumors were clearly delineated from normal tissue with tumor-to-background ratios of 2.9(Pearl) and 2.3(SPY). Disease detection was significantly improved with panitumumab-IRDye compared to IgG-IRDye800 (P<0.05). Tissue biopsies (average size=3.7mm) positive for fluorescence were confirmed for pathologic disease by histology and immunohistochemistry (n=25/25). Biopsies of non-fluorescent tissue were proven to be negative for malignancy (n=28/28). The SPY was able to detect regional lymph node metastasis (<1.0mm) and microscopic areas of disease. Standard histological assessment in both frozen and paraffin-embedded histologic specimens was augmented using the Odyssey. Panitumumab-IRDye800 may have clinical utility in detection and removal of microscopic HNSCC using existing intraoperative optical imaging hardware and may augment analysis of frozen and permanent pathology.
登录
查看更多内容
影响因子：
3.1
作者：
Marshall, Milton V.;Draney, Daniel;Sevick-Muraca, Eva M.;Olive, D. Michael
通讯作者：
Olive, D. Michael
影响因子：
3.1
作者：
Keereweer, Stijn;Kerrebijn, Jeroen D. F.;van Driel, Pieter B. A. A.;Xie, Bangwen;Kaijzel, Eric L.;Snoeks, Thomas J. A.;Que, Ivo;Hutteman, Merlijn;van der Vorst, Joost R.;Mieog, J. Sven D.;Vahrmeijer, Alexander L.;van de Velde, Cornelis J. H.;de Jong, Robert J. Baatenburg;Lowik, Clemens W. G. M.
通讯作者：
Lowik, Clemens W. G. M.
影响因子：
3.4
作者：
Withrow, Kirk P.;Gleysteen, John P.;Rosenthal, Eben L.
通讯作者：
Rosenthal, Eben L.
DOI：
10.1002/hed.20782
发表时间：
2008-06-01
影响因子：
2.9
作者：
Gleysteen, John P.;Newman, J. Robert;Rosenthal, Eben L.
通讯作者：
Rosenthal, Eben L.
影响因子：
82.9
作者：
van Dam, Gooitzen M.;Themelis, George;Ntziachristos, Vasilis
通讯作者：
Ntziachristos, Vasilis