Virus-induced diabetes mellitus. XVIII. Inhibition by a nondiabetogenic variant of encephalomyocarditis virus.
Virus-induced diabetes mellitus. XVIII. Inhibition by a nondiabetogenic variant of encephalomyocarditis virus.
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DOI：
10.1084/jem.152.4.878
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发表时间：
1980-10-01
期刊：
影响因子：
--
通讯作者：
Notkins AL
中科院分区：
文献类型：
--
作者：
Yoon JW;McClintock PR;Onodera T;Notkins AL
对脑心肌炎（EMC）病毒的M变异体进行空斑纯化，分离出两种稳定的变异体：一种是致糖尿病的，命名为D，另一种是非致糖尿病的，命名为B。当D变体接种到SJL/J雄性小鼠中时，在> 90%的动物中出现低胰岛素血症和高血糖症。相比之下，接种B变异体的小鼠没有一只发生糖尿病。对感染D变体的小鼠胰腺进行组织学检查，发现胰岛炎和β细胞坏死，而感染B变体的小鼠胰岛几乎没有变化。当检测胰岛的感染性病毒时，与B变体相比，从接种D变体的动物中回收的病毒大约多10倍。此外，当用荧光素标记的抗EMC病毒抗体染色时，感染D变体的小鼠的胰岛细胞中约60%含有病毒抗原，而感染B变体的动物的胰岛细胞中< 5%含有病毒抗原。共感染实验表明，D变异体诱导糖尿病可被B变异体抑制。当B和D变体以1、9和99的B：D比例混合在一起时，分别有60、11和0%的小鼠发生糖尿病。组织培养实验表明，B变体诱导的干扰素比D变体多得多，动物研究表明，与D变体相比，在感染B变体的小鼠的循环中，干扰素出现得更早，量更大。这些研究表明，B变体对干扰素的诱导至少部分地负责D变体对糖尿病的抑制。
Plaque purification of the M variant of encephalomyocarditis (EMC) virus resulted in the isolation of two stable variants: one diabetogenic and designated D and the other nondiabetogenic and designated B. When the D variant was inoculated into SJL/J male mice, hypoinsulinemia and hyperglycemia developed in > 90% of the animals. In contrast, none of the mice inoculated with the B variant developed diabetes. Histologic examination of pancreata from mice infected with the D variant revealed insulitis and necrosis of beta cells, whereas islets from mice infected with the B variant showed little, if any, change. When islets were assayed for infectious virus, approximately 10 times more virus was recovered from animals inoculated with the D as compared with the B variant. Moreover, approximately 60% of islet cells from mice infected with the D variant contained viral antigens when stained with fluorescein-labeled anti-EMC virus antibody, whereas < 5% of islet cells from animals infected with the B variant contained viral antigens. Co-infection experiments showed that the induction of diabetes by the D variant was inhibited by the B variant. When the B and D variants were mixed together at B:D ratios of 1, 9, and 99, diabetes developed in 60, 11, and 0% of the mice, respectively. Tissue- culture experiments revealed that the B variant induced considerably more interferon than the D variant, and studies in animals showed that interferon appeared earlier and in greater amounts in the circulation of mice infected with the B as compared with the D variant. These studies suggest that the induction of interferon by the B variant is, at least in part, responsible for the inhibition of diabetes by the D variant.
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影响因子：
4.1
作者：
HALES, CN;RANDLE, PJ
通讯作者：
RANDLE, PJ
影响因子：
15.3
作者：
YOON, JW;NOTKINS, AL
通讯作者：
NOTKINS, AL
影响因子：
15.3
作者：
BOUCHER, DW;NOTKINS, AL
通讯作者：
NOTKINS, AL
影响因子：
64.8
作者：
HALLER, O;ARNHEITER, H;GRESSER, I
通讯作者：
GRESSER, I
影响因子：
7.7
作者：
ROSS, ME;ONODERA, T;NOTKINS, AL
通讯作者：
NOTKINS, AL