Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY.
Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY.
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基本的螺旋 - 环螺旋转录因子TCF21是确定基因SRY的男性性别的下游靶标。
DOI：
10.1371/journal.pone.0019935
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Skinner MK
中科院分区：
文献类型：
--
作者：
Bhandari RK;Sadler-Riggleman I;Clement TM;Skinner MK
哺乳动物性别决定中的级联分子事件已被证明涉及SRY基因，但几十年来研究人员一直没有发现特定的下游事件。目前的研究确定了男性性别决定因子SRY的第一个直接下游靶点之一为碱性螺旋环螺旋（bHLH）转录因子TCF 21。发现SRY结合Tcf 21启动子并激活基因表达。Tcf 21启动子中SRY/SOX 9应答元件的突变消除了SRY的作用。在胚胎大鼠睾丸发育过程中，SRY被发现与Tcf 21启动子SRY/SOX 9反应元件直接相关。发现TCF 21促进胚胎卵巢细胞的体外性逆转以诱导前体支持细胞分化。TCF 21和SRY对体外性反转性腺细胞转录组的影响相似。因此，SRY直接作用于Tcf 21启动子，部分启动与支持细胞分化和胚胎睾丸发育相关的级联反应。
The cascade of molecular events involved in mammalian sex determination has been shown to involve the SRY gene, but specific downstream events have eluded researchers for decades. The current study identifies one of the first direct downstream targets of the male sex determining factor SRY as the basic-helix-loop-helix (bHLH) transcription factor TCF21. SRY was found to bind to the Tcf21 promoter and activate gene expression. Mutagenesis of SRY/SOX9 response elements in the Tcf21 promoter eliminated the actions of SRY. SRY was found to directly associate with the Tcf21 promoter SRY/SOX9 response elements in vivo during fetal rat testis development. TCF21 was found to promote an in vitro sex reversal of embryonic ovarian cells to induce precursor Sertoli cell differentiation. TCF21 and SRY had similar effects on the in vitro sex reversal gonadal cell transcriptomes. Therefore, SRY acts directly on the Tcf21 promoter to in part initiate a cascade of events associated with Sertoli cell differentiation and embryonic testis development.
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影响因子：
3.8
作者：
Clement, Tracy M.;Anway, Matthew D.;Skinner, Michael K.
通讯作者：
Skinner, Michael K.
影响因子：
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影响因子：
4.8
作者：
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通讯作者：
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