Antifungal activity of pregnane glycosides isolated from Periploca sepium root barks against various phytopathogenic fungi
Antifungal activity of pregnane glycosides isolated from Periploca sepium root barks against various phytopathogenic fungi
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杠柳根皮中分离的孕烷苷对多种植物病原真菌的抗真菌活性
DOI：
10.1016/j.indcrop.2019.02.009
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发表时间：
2019-06
影响因子：
5.9
通讯作者：
Wu Wenjun
中科院分区：
文献类型：
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作者：
Li Yankai;Aioub Ahmed A A;Lv Bo;Hu Zhaonong;Wu Wenjun
毫无疑问，研究植物产生的次级代谢产物可以发现新的农药。本研究采用生物活性测定法从杠柳根皮中分离活性成分，并对7种植物病原真菌进行了体外抑菌活性验证。从该植物中分离得到6个甾烷苷类化合物，分别鉴定为杠柳苷A（PSA）、杠柳苷D（PSD）、杠柳苷E（PSE）、杠柳苷F（PSF）、杠柳苷N（PSN）和杠柳苷W（PSW）。生物测定结果表明，PSW对苹果腐烂病菌和禾谷镰刀菌具有极显著的抑菌活性，EC 50分别为26.50 mg/L和93.94 mg/L。PSW（1000 mg/L）对苹果腐烂病的防效为85.40%，显著高于多菌灵（200 mg/L）的防效64.62%。两组疗效无显著性差异，分别为71.32%和69.70%。因此，本研究建议将PSRB中的甾烷苷类化合物作为一类新的杀菌剂或杀菌剂先导化合物，不仅因为其潜在的抗真菌活性，而且因为其对人、哺乳动物和非靶标生物的安全性。
No doubt that studying the secondary metabolites produced by plants can lead to the detection of new pesticides. In this study, we followed the bioassay-guided method to isolate active ingredients fromPeriploca sepiumroot barks (PSRB) and verified their antifungal activity against seven phytopathogenic fungiin vitro. A total of six pregnane glycosides were isolated and identified as periplocoside A (PSA), periplocoside D (PSD), periplocoside E (PSE), periplocoside F (PSF), periplocoside N (PSN) and periploside W (PSW). Bioassay results revealed that PSW exhibited highly significant antifungal activity againstValsa maliandFusarium graminearumwith EC50values of 26.50 mg/L and 93.94 mg/L, respectively. In controlling apple Valsa canker, PSW (1000 mg/L) exhibited significantly higher protective activity with 85.40% than that efficacy with 64.62% obtained by carbendazim (200 mg/L). Whereas there is no significant difference between both of them in the curative efficacy with 71.32% and 69.70%, respectively. In conclusion, our study recommended the pregnane glycosides from PSRB as a new class of fungicides or fungicide lead compounds not only because of their potential antifungal activity, but also because of their safety for human beings, mammals and non-target organisms.
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DOI：
--
发表时间：
2006
期刊：
--
影响因子：
--
作者：
Shi Qing-hua;Qin Hu-qiang
通讯作者：
Shi Qing-hua;Qin Hu-qiang
影响因子：
3.2
作者：
E. Jeong;Myung-Ji Lee;Min Kang;Hoi-Seon Lee
通讯作者：
E. Jeong;Myung-Ji Lee;Min Kang;Hoi-Seon Lee
影响因子：
5.1
作者：
Luoyi Wang;Jun-jun Qin;Zhen-hua Chen;Yu Zhou;Wei Tang;Jian-Ping Zuo;Weimin Zhao
通讯作者：
Luoyi Wang;Jun-jun Qin;Zhen-hua Chen;Yu Zhou;Wei Tang;Jian-Ping Zuo;Weimin Zhao
影响因子：
1.7
作者：
S. Kawanishi;S. Sakuma;J. Shoji
通讯作者：
S. Kawanishi;S. Sakuma;J. Shoji
DOI：
10.3390/molecules15085807
发表时间：
2010-08-24
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Wang J;Liu H;Zhao J;Gao H;Zhou L;Liu Z;Chen Y;Sui P
通讯作者：
Sui P