Antimicrobial and antioxidant activities of the root bark essential oil of Periploca sepium and its main component 2-hydroxy-4-methoxybenzaldehyde.
Antimicrobial and antioxidant activities of the root bark essential oil of Periploca sepium and its main component 2-hydroxy-4-methoxybenzaldehyde.
复制标题
DOI：
10.3390/molecules15085807
复制
发表时间：
2010-08-24
期刊：
影响因子：
--
通讯作者：
Sui P
中科院分区：
文献类型：
--
作者：
Wang J;Liu H;Zhao J;Gao H;Zhou L;Liu Z;Chen Y;Sui P
采用气相色谱和气相色谱-质谱联用技术对加氢蒸馏法提取的麻麻根皮精油进行了研究。鉴定出的17种化合物中以2-羟基-4-甲氧基苯甲醛为主要成分，占总成分的78.8%。采用制备薄层色谱法对2-羟基-4-甲氧基苯甲醛进行分离纯化，并通过理化和光谱分析对其进行鉴定。精油和2-羟基-4-甲氧基苯甲醛对所有试验细菌和真菌均表现出抑菌活性，MBC/MFC值为125 ~ 300 μg/mL， MIC值为80 ~ 300 μg/mL， IC50值为63.29 ~ 167.30 μg/mL。它们在DPPH自由基清除、β-胡萝卜素漂白和亚铁-铁离子测定中也显示出中等的抗氧化活性。结果表明，这些抗菌和抗氧化活性的主要原因是油中含有2-羟基-4-甲氧基苯甲醛，具有开发利用的潜力。
The root bark essential oil of Periploca sepium Bunge (Asclepiadaceae/ Apocynaceae) obtained by hydrodistillation was investigated by GC and GC-MS. 2-Hydroxy-4-methoxybenzaldehyde was found to be the main component (78.8% of the total) among 17 identified compounds. 2-Hydroxy-4-methoxybenzaldehyde was separated and purified by preparative thin layer chromatography, and was further identified by means of physicochemical and spectrometric analysis. Both the essential oil and 2-hydroxy-4-methoxybenzaldehyde exhibited antimicrobial activities on all test bacteria and fungi, with MBC/MFC values ranging from 125 μg/mL to 300 μg/mL, MIC values from 80 μg/mL to 300 μg/mL, and IC50 values from 63.29 μg/mL to 167.30 μg/mL. They also showed moderate antioxidant activity in the DPPH free radical scavenging, β-carotene bleaching and ferrozine-ferrous ions assays. The results indicated that the major portion of these antimicrobial and antioxidant activities was due to the presence of 2-hydroxy-4-methoxy-benzaldehyde in the oil, which could potentially be developed as an antimicrobial and antioxidant agent in the future.
登录
查看更多内容
影响因子：
1.3
作者：
Sakuma, M
通讯作者：
Sakuma, M
影响因子：
2.7
作者：
Sreekumar, S;Seeni, S;Pushpangadan, P
通讯作者：
Pushpangadan, P
影响因子：
8.8
作者：
Hajji, Mohamed;Masmoudi, Ons;Nasri, Moncef
通讯作者：
Nasri, Moncef
影响因子：
2.9
作者：
Abe, K;Matsuki, N
通讯作者：
Matsuki, N
影响因子：
3.3
作者：
Magina, Michele D. A.;Dalmarco, Eduardo M.;Brighente, Ines M. C.
通讯作者：
Brighente, Ines M. C.