Effects of blue or violet light on the inactivation of Staphylococcus aureus by riboflavin-5'-phosphate photolysis.
Effects of blue or violet light on the inactivation of Staphylococcus aureus by riboflavin-5'-phosphate photolysis.
复制标题
蓝光或紫光对核黄素-5-磷酸光解灭活金黄色葡萄球菌的影响。
DOI：
10.1016/j.jphotobiol.2017.07.009
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Ji
中科院分区：
文献类型：
--
作者：
Tak;Chien;Zong;Ji
光敏化合物核黄素-5 ′-磷酸（或黄素单酰肽，FMN）在光照射时产生活性氧（ROS）。FMN是所有黄素蛋白所必需的，因为它是生物蓝光受体的辅因子。研究了FMN经蓝光或紫光照射后对金黄色葡萄球菌（包括耐甲氧西林金黄色葡萄球菌（MRSA））的光化学灭活作用。在蓝光或紫光光处理后，FMN显示出失活S。由于产生的ROS，FMN光解可以在没有外源电子提供者的情况下实现有效的细菌灭活。120 μM FMN在2.0mW/cm ~ 2蓝光照射120 min，对金黄色葡萄球菌和MRSA的灭活率分别为94.9%和95.2%。较低的FMN浓度和较短的时间需要通过紫光照射达到类似的效果。用30 μM的FMN在1.0mW/cm ~ 2的紫光照射30 min，对金黄色葡萄球菌和MRSA的灭活率分别为96.3%和97.0%。在蓝光照射下，固有光敏剂的灵敏度较低。需要通过蓝光对FMN进行长时间曝光光解处理以使其失活。金黄色。在相同的辐射强度下，紫光灭活金黄色葡萄球菌的效率更高。FMN在蓝光或紫光照射下的光解均能提高S.金黄色葡萄球菌和MRSA。FMN光化学处理可作为卫生去污工艺的一种补充技术。
The light sensitive compound riboflavin-5′-phosphate (or flavin mononucleotide, FMN) generates reactive oxygen species (ROS) upon photo-irradiation. FMN is required by all flavoproteins because it is a cofactor of biological blue-light receptors. The photochemical effects of FMN after irradiation by blue or violet light on the inactivation ofStaphylococcus aureusstrains, including a methicillin-resistant strain (MRSA), were investigated in this study. Upon blue- or violet-light photo-treatment, FMN was shown to inactivateS. aureusdue to the generated ROS. Effective bacterial inactivation can be achieved by FMN photolysis without an exogenous electron provider. Inactivation rates of 94.9 and 95.2% inS. aureusand MRSA, respectively, can be reached by blue light irradiation (2.0 mW/cm2) with 120 μM FMN for 120 min. A lower FMN concentration and a shorter time are required to reach similar effects by violet light irradiation. Inactivation rates of 96.3 and 97.0% inS. aureusand MRSA, respectively, can be reached by violet light irradiation (1.0 mW/cm2) with 30 μM FMN for 30 min. The sensitivity of the inherent photosensitizers is lower under blue-light irradiation. A long exposure photolytic treatment of FMN by blue light is required to inactivateS. aureus. Violet light was found to be more efficient inS. aureusinactivation at the same radiant intensity. FMN photolysis with blue or violet light irradiation enhanced the inactivation rates ofS. aureusand MRSA. FMN photochemical treatment could be a supplemental technique in hygienic decontamination processes.
影响因子：
3.7
作者：
Maisch T;Eichner A;Späth A;Gollmer A;König B;Regensburger J;Bäumler W
通讯作者：
Bäumler W
基于光的抗感染物：紫外线C辐射，光动力疗法，蓝光等。
影响因子：
4
作者：
Yin, Rui;Dai, Tianhong;Avci, Pinar;Serafim Jorge, Ana Elisa;de Melo, Wanessa C. M. A.;Vecchio, Daniela;Huang, Ying-Ying;Gupta, Asheesh;Hamblin, Michael R.
通讯作者：
Hamblin, Michael R.