Fast and effective photodynamic inactivation of multiresistant bacteria by cationic riboflavin derivatives.
Fast and effective photodynamic inactivation of multiresistant bacteria by cationic riboflavin derivatives.
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DOI：
10.1371/journal.pone.0111792
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bäumler W
中科院分区：
文献类型：
--
作者：
Maisch T;Eichner A;Späth A;Gollmer A;König B;Regensburger J;Bäumler W
细菌光动力灭活（PIB）被证明是杀灭病原菌的另一种方法。PIB需要光敏剂分子，其在暴露于可见光时有效地产生活性氧物质如单线态氧。为了允许在医学中的广泛应用，光敏剂在应用于人类时应该是安全的。像维生素B2这样的物质，最有可能是安全的，已知在辐射时会产生单线态氧。在本研究中，我们向黄素衍生物中加入正电荷，使这些分子能够附着在带负电荷的细菌表面。两个合成的黄素衍生物显示出约75%的高量子产率的单线态氧。将多重耐药细菌如MRSA（耐甲氧西林金黄色葡萄球菌）、EHEC（肠出血性大肠杆菌）、铜绿假单胞菌和鲍曼不动杆菌与这些黄素衍生物在体外孵育，随后仅用可见光照射数秒。通过在1270 nm处检测其发光，证明细菌中产生单线态氧。照射后，根据黄素衍生物的浓度和光剂量测定法，活菌的数量下降至6 log10步骤。PIB的杀菌效果与细菌类型和相应的抗生素耐药模式无关。相比之下，用于杀菌的光敏剂浓度和光参数不影响人角质形成细胞的细胞活力（治疗窗）。多重耐药细菌可以通过修饰的维生素B2分子，氧气和可见光的组合安全有效地杀死，而正常皮肤细胞则存活。进一步的工作将包括这些新的光敏剂局部应用，从皮肤和粘膜去殖民化细菌。
Photodynamic inactivation of bacteria (PIB) proves to be an additional method to kill pathogenic bacteria. PIB requires photosensitizer molecules that effectively generate reactive oxygen species like singlet oxygen when exposed to visible light. To allow a broad application in medicine, photosensitizers should be safe when applied in humans. Substances like vitamin B2, which are most likely safe, are known to produce singlet oxygen upon irradiation. In the present study, we added positive charges to flavin derivatives to enable attachment of these molecules to the negatively charged surface of bacteria. Two of the synthesized flavin derivatives showed a high quantum yield of singlet oxygen of approximately 75%. Multidrug resistant bacteria like MRSA (Methicillin resistant Staphylococcus aureus), EHEC (enterohemorrhagic Escherichia coli), Pseudomonas aeruginosa, and Acinetobacter baumannii were incubated with these flavin derivatives in vitro and were subsequently irradiated with visible light for seconds only. Singlet oxygen production in bacteria was proved by detecting its luminescence at 1270 nm. After irradiation, the number of viable bacteria decreased up to 6 log10 steps depending on the concentration of the flavin derivatives and the light dosimetry. The bactericidal effect of PIB was independent of the bacterial type and the corresponding antibiotic resistance pattern. In contrast, the photosensitizer concentration and light parameters used for bacteria killing did not affect cell viability of human keratinocytes (therapeutic window). Multiresistant bacteria can be safely and effectively killed by a combination of modified vitamin B2 molecules, oxygen and visible light, whereas normal skin cells survive. Further work will include these new photosensitizers for topical application to decolonize bacteria from skin and mucosa.
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通过阳离子中二取代的卟啉对革兰氏阴性和革兰氏阳性细菌的光活化的电荷效应。
影响因子：
4.2
作者：
Alves E;Costa L;Carvalho CM;Tomé JP;Faustino MA;Neves MG;Tomé AC;Cavaleiro JA;Cunha A;Almeida A
通讯作者：
Almeida A
影响因子：
4.2
作者：
Garcez, Aguinaldo Silva;Nunez, Silvia Cristina;Ribeiro, Martha Simoes
通讯作者：
Ribeiro, Martha Simoes
DOI：
10.1126/science.1176667
发表时间：
2009-08-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fischbach MA;Walsh CT
通讯作者：
Walsh CT
影响因子：
6.4
作者：
Hoboth, Christina;Hoffmann, Reinhard;Hogardt, Michael
通讯作者：
Hogardt, Michael
DOI：
10.3390/v5010015
发表时间：
2012-12-21
期刊：
Viruses
影响因子：
--
作者：
Krylov V;Shaburova O;Krylov S;Pleteneva E
通讯作者：
Pleteneva E