Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening.
Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening.
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DOI：
10.1186/1471-2164-9-351
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发表时间：
2008-07-27
期刊：
影响因子：
4.4
通讯作者：
Laing WA
中科院分区：
文献类型：
--
作者：
Crowhurst RN;Gleave AP;MacRae EA;Ampomah-Dwamena C;Atkinson RG;Beuning LL;Bulley SM;Chagne D;Marsh KB;Matich AJ;Montefiori M;Newcomb RD;Schaffer RJ;Usadel B;Allan AC;Boldingh HL;Bowen JH;Davy MW;Eckloff R;Ferguson AR;Fraser LG;Gera E;Hellens RP;Janssen BJ;Klages K;Lo KR;MacDiarmid RM;Nain B;McNeilage MA;Rassam M;Richardson AC;Rikkerink EH;Ross GS;Schröder R;Snowden KC;Souleyre EJ;Templeton MD;Walton EF;Wang D;Wang MY;Wang YY;Wood M;Wu R;Yauk YK;Laing WA
猕猴桃（Actinidia spp.）是一种相对较新的，但在世界许多不同地区种植的经济上重要的作物。商业上的成功是由具有新的消费者特征的新品种的开发驱动的，包括风味、外观、健康成分和便利性。为了增加我们对猕猴桃遗传多样性和这些关键性状的基因控制的理解，我们收集了132，577个表达序列标签（EST）。这些EST主要来源于猕猴桃属的四个种（A. chinensis，中国蒿A. deliciosa、细叶甲A. A.tagata和A. Eriantha），并落入41，858个非冗余簇（18，070个暂定共有序列和23，788个EST单例）。风味和香味相关的基因家族（酰基转移酶和羧酸酯酶）和途径（萜类化合物的生物合成）的分析提出了比较与猕猴桃中存在的化合物，包括酯，酸，醇和萜类化合物的化学分析。EST被鉴定为控制叶绿素降解和类胡萝卜素生物合成的颜色途径中的大多数基因。在健康领域，数据的EST参与抗坏血酸和奎尼酸的生物合成，不仅显示在这些途径中的许多步骤的基因在数据库中表示，但基因编码的一些关键步骤是缺席。在便利区域，鉴定了与果实软化的不同阶段相关的基因。这一大型EST资源将使研究人员能够承担理解猕猴桃属遗传多样性的分子基础的巨大挑战，并为比较水果基因组学提供EST资源。我们已经进行的各种生物信息学分析表明了猕猴桃中编码不同生化途径的基因的EST的覆盖范围。
Kiwifruit (Actinidia spp.) are a relatively new, but economically important crop grown in many different parts of the world. Commercial success is driven by the development of new cultivars with novel consumer traits including flavor, appearance, healthful components and convenience. To increase our understanding of the genetic diversity and gene-based control of these key traits in Actinidia, we have produced a collection of 132,577 expressed sequence tags (ESTs). The ESTs were derived mainly from four Actinidia species (A. chinensis, A. deliciosa, A. arguta and A. eriantha) and fell into 41,858 non redundant clusters (18,070 tentative consensus sequences and 23,788 EST singletons). Analysis of flavor and fragrance-related gene families (acyltransferases and carboxylesterases) and pathways (terpenoid biosynthesis) is presented in comparison with a chemical analysis of the compounds present in Actinidia including esters, acids, alcohols and terpenes. ESTs are identified for most genes in color pathways controlling chlorophyll degradation and carotenoid biosynthesis. In the health area, data are presented on the ESTs involved in ascorbic acid and quinic acid biosynthesis showing not only that genes for many of the steps in these pathways are represented in the database, but that genes encoding some critical steps are absent. In the convenience area, genes related to different stages of fruit softening are identified. This large EST resource will allow researchers to undertake the tremendous challenge of understanding the molecular basis of genetic diversity in the Actinidia genus as well as provide an EST resource for comparative fruit genomics. The various bioinformatics analyses we have undertaken demonstrates the extent of coverage of ESTs for genes encoding different biochemical pathways in Actinidia.
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