Applications for multifrequency ultrasound biomicroscopy in mice from implantation to adulthood.
Applications for multifrequency ultrasound biomicroscopy in mice from implantation to adulthood.
复制标题
多频超声生物显微镜在小鼠从着床到成年期间的应用。
作者：
Yu;Yu;F. Foster;F. Foster;D. Qu;M. Y. Zhang;M. Y. Zhang;K. Harasiewicz;K. Harasiewicz;S. Adamson;S. Adamson
一种新的多频（19-55 MHz）超声生物显微镜与二维成像和集成多普勒超声进行了评估使用phanomaly和异氟烷麻醉小鼠。结果显示，生物显微镜的横向分辨率在50至100微米之间，而传统的13 MHz超声系统的横向分辨率为200-500微米。这种差异在使用生物显微镜在体内获得的分辨率明显更高的图像中是明显的。使用25至40 MHz的频率获得了植入后约2天（妊娠7天）至近足月（17.5天）妊娠小鼠胚胎的经皮图像。外胎盘锥和早期胚胎腔是可见的，因为是胎盘和胚胎器官在整个发展到足月。我们还评估了生物显微镜的能力，以检测心脏发育的重要特征，通过检查胚胎从8.5至17.5天的妊娠在exteriorized子宫使用55 MHz的超声。可见心脏成环、流出道分裂和心室间隔。在出生后成像中，我们在55 MHz下观察了新生小鼠的心脏和肾脏，在40 MHz下观察了青少年（约8 g体重）和成年人（约25 g体重）的颈动脉，在19 MHz下观察了成年人的心脏、主动脉和肾脏。颈动脉和主动脉直径测量的变异系数为1- 3%。此外，GRIP 1-/-胚胎中的水泡和两个成人中的主动脉瓣狭窄很容易观察到。使用图像引导的多普勒功能，在直径小至100微米的血管（包括第8.5天的原始心管）中的低血流速度是可测量的，但是在妊娠后期和出生后的生命中的心脏和大动脉中的高血流速度（> 37.5cm/s）是不合比例的。时间分辨率差（4帧/秒）阻碍了准确的心脏尺寸测量。总之，多频超声生物显微镜是一种多功能的工具，非常适合于详细研究小鼠整个发育过程中各种器官系统的形态，并在低速范围内进行血液动力学测量。
A new multifrequency (19-55 MHz) ultrasound biomicroscope with two-dimensional imaging and integrated Doppler ultrasound was evaluated using phantoms and isoflurane-anesthetized mice. Phantoms revealed the biomicroscope's lateral resolution was between 50 and 100 microm, whereas that of a conventional 13 MHz ultrasound system was 200-500 microm. This difference was apparent in the markedly higher resolution images achieved using the biomicroscope in vivo. Transcutaneous images of embryos in pregnant mice from approximately 2 days after implantation (7 days gestation) to near term (17.5 days) were obtained using frequencies from 25 to 40 MHz. The ectoplacental cone and early embryonic cavities were visible as were the placenta and embryonic organs throughout development to term. We also evaluated the ability of the biomicroscope to detect important features of heart development by examining embryos from 8.5 to 17.5 day gestation in exteriorized uteri using 55 MHz ultrasound. Cardiac looping, division of the outflow tract, and ventricular septation were visible. In postnatal imaging, we observed the heart and kidney of neonatal mice at 55 MHz, the carotid artery in juveniles (approximately 8 g body wt) and adults (approximately 25 g body wt) at 40 MHz, and the adult heart, aorta, and kidney at 19 MHz. The coefficient of variation of carotid and aortic diameter measurements was 1-3%. In addition, blisters in GRIP1 -/- embryos and aortic valvular stenosis in two adults were readily visualized. Using image-guided Doppler function, low blood velocities in vessels as small as 100 microm in diameter including the primitive heart tube at day 8.5 were measurable, but high blood velocities (>37.5 cm/s) such as in the heart and large arteries in late gestation and postnatal life were off-scale. Accurate cardiac dimension measurements were impeded by poor temporal resolution (4 frames/s). In summary, the multifrequency ultrasound biomicroscope is a versatile tool well suited to detailed study of the morphology of various organ systems throughout development in mice and for hemodynamic measurements in the low velocity range.
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DOI：
10.1152/ajpheart.1999.277.5.h1967
发表时间：
1999-11-01
影响因子：
4.8
作者：
Yang, XP;Liu, YH;Carretero, OA
通讯作者：
Carretero, OA
DOI：
10.1016/s0895-6111(98)00060-3
发表时间：
1999
期刊：
Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society
影响因子：
--
作者：
D. Turnbull
通讯作者：
D. Turnbull
使用多普勒超声心动图监测胚胎小鼠心脏功能。
DOI：
10.1385/1-59259-685-1:245
发表时间：
2000
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Linask,KK;Huhta,JC
通讯作者：
Huhta,JC
DOI：
10.1152/ajpheart.1996.270.6.h2204
发表时间：
1996-06
期刊：
The American journal of physiology
影响因子：
--
作者：
G. Taffet;C. Hartley;Xiouying Wen;T. Pham;L. Michael;M. Entman
通讯作者：
G. Taffet;C. Hartley;Xiouying Wen;T. Pham;L. Michael;M. Entman
小鼠胚胎的超声背散射显微镜。
DOI：
10.1385/1-59259-685-1:235
发表时间：
2000
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Turnbull,DH
通讯作者：
Turnbull,DH