APEX1/miR-24 axis: a promising therapeutic target in endometriosis
APEX1/miR-24 axis: a promising therapeutic target in endometriosis
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APEX1/miR-24轴：子宫内膜异位症的一个有前途的治疗靶点
DOI：
10.1007/s00404-021-05963-6
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发表时间：
2021-01
影响因子：
2.6
通讯作者：
Sun Pengxing
中科院分区：
文献类型：
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作者：
Tan Aili;Ruan Peng;Sun Pengxing
摘要。目的探讨APEX1在子宫内膜间质细胞（ESC）和子宫内膜间质细胞中的异常表达。方法采用qRT-PCR和Western blot检测子宫内膜异位症组织中APEX1和miR-24的表达水平。在细胞之后。转染后，细胞相应分为pcDNA3.1-NC、sh-NC、mimic NC、inhibitor NC、pcDNA3.1-APEX1、.sh-APEX1、miR-24 mimic、miR-24 inhibitor、sh-NC + inhibitor NC、inhibitor-NC + sh-APEX1、sh-NC + miR-24 inhibitor-。pcDNA3.1-NC + mimic NC、mimic NC + pcDNA3.1-APEX1和pcDNA3.1-NC + miR-24 mimic组。此外，细胞。BrdU分析细胞增殖、凋亡及凋亡相关蛋白Bax、Bcl-2和cleaved-casase-3。流式细胞术（FCM）和Western blot检测。此外，通过RIP测定pri-miR-24和miR-24之间的相互作用。结果APEX1和miR-24在子宫内膜异位症组织中高表达。APEX1和miR-24电位过表达。抑制ESC增殖和细胞凋亡，而这些作用可以通过APEX1和miR-24沉默逆转。同时，APEX1和miR-24可上调ESC细胞凋亡相关蛋白Bax和cleaved-caspase-3的表达，降低Bcl-2的表达。结论APEX1通过上调miR-24的表达促进ESC细胞增殖，抑制细胞凋亡
Abstract.Purpose The present work aimed to explore the aberrant expression of APEX1 in endometrial stromal cells (ESC) and the .underlying mechanisms..Methods The levels of APEX1 and miR-24 in endometriosis tissues were tested by qRT-PCR and Western blot. After cell .transfection, cells were correspondingly classified into pcDNA3.1-NC, sh-NC, mimic NC, inhibitor NC, pcDNA3.1-APEX1, .sh-APEX1, miR-24 mimic, miR-24 inhibitor, sh-NC + inhibitor NC, inhibitor-NC + sh-APEX1, sh-NC + miR-24 inhibi-.tor, pcDNA3.1-NC + mimic NC, mimic NC + pcDNA3.1-APEX1 and pcDNA3.1-NC + miR-24 mimic group. Besides, cell .proliferation, apoptosis in addition to apoptosis-related proteins Bax, Bcl-2 and cleaved-casase-3 were analyzed by BrdU .assay, flow cytometry (FCM) and Western blot assays, respectively. Additionally, RIP assay was conducted to determine the .interaction between pri-miR-24 and miR-24..Results APEX1 and miR-24 were highly expressed in endometriosis tissues. Overexpression of APEX1 and miR-24 poten-.tiates ESC proliferation and inhibits apoptosis, while those effects could be reversed by APEX1 and miR-24 silencing. .Meanwhile, APEX1 and miR-24 could elevate ESC apoptosis-related proteins Bax and cleaved-caspase-3 and decrease Bcl-2 .expression. Importantly, APEX1 was positively correlated with miR-24 expression..Conclusion APEX1 promotes ESC proliferation and inhibits apoptosis by upregulating miR-24 expression
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影响因子：
--
作者：
Dong P;Ihira K;Xiong Y;Watari H;Hanley SJ;Yamada T;Hosaka M;Kudo M;Yue J;Sakuragi N
通讯作者：
Sakuragi N
影响因子：
5.6
作者：
L. Villanova;Chiara Barbini;Cristina Piccolo;Alessandra Boe;R. De Maria;M. Fiori
通讯作者：
L. Villanova;Chiara Barbini;Cristina Piccolo;Alessandra Boe;R. De Maria;M. Fiori
影响因子：
3.9
作者：
Kim, Ji-Myung;Yeo, Min-Kyung;Kim, Kyung-Hee
通讯作者：
Kim, Kyung-Hee
影响因子：
6.1
作者：
Cai H;Zhu XX;Li ZF;Zhu YP;Lang JH
通讯作者：
Lang JH
DOI：
10.1158/1541-7786.mcr-16-0218
发表时间：
2017-07
期刊：
Molecular cancer research : MCR
影响因子：
--
作者：
Ballista-Hernández J;Martínez-Ferrer M;Vélez R;Climent C;Sánchez-Vázquez MM;Torres C;Rodríguez-Muñoz A;Ayala-Peña S;Torres-Ramos CA
通讯作者：
Torres-Ramos CA