Transcriptional profiles of drought-responsive genes in modulating transcription signal transduction, and biochemical pathways in tomato.
Transcriptional profiles of drought-responsive genes in modulating transcription signal transduction, and biochemical pathways in tomato.
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番茄中干旱响应基因调节转录信号转导和生化途径的转录谱
DOI：
10.1093/jxb/erq167
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发表时间：
2010-08
影响因子：
6.9
通讯作者：
Ye Z
中科院分区：
文献类型：
--
作者：
Gong P;Zhang J;Li H;Yang C;Zhang C;Zhang X;Khurram Z;Zhang Y;Wang T;Fei Z;Ye Z
为了揭示番茄干旱反应的分子机制，本研究以两个番茄耐旱品系及其轮回亲本番茄为材料，研究了它们的基因表达谱。利用番茄基因芯片对干旱胁迫下的敏感品种M82进行了研究。已鉴定的大约400个基因只在耐旱系中对干旱胁迫做出反应。这些基因表达的变化很可能是由彭尼利链霉菌插入的两个染色体片段引起的，这些染色体片段可能含有耐旱性数量性状基因座(QTL)。在这些基因中有许多转录因子和信号蛋白，它们可能是参与番茄对干旱胁迫反应的全球调节因子。参与生物体生长发育过程的基因也受到干旱胁迫的特异性调控，包括那些控制细胞壁结构、蜡质生物合成和植株高度的基因。此外，糖异生途径中的关键酶(果糖-二磷酸缩醛酶)、嘌呤和嘧啶核苷酸生物合成途径(腺苷酸激酶)、色氨酸降解途径(醛氧化酶)、淀粉降解途径(β-淀粉酶)、蛋氨酸生物合成途径(胱硫氨酸β-裂解酶)和超氧阴离子自由基清除途径(过氧化氢酶)也受到干旱胁迫的专一性影响。这些结果表明，番茄植株可以通过减少能量消耗、增加ATP能量供应、减少氧化损伤来适应水分亏缺条件。本研究中发现的干旱反应基因可以为了解番茄的耐旱机制提供进一步的信息。
To unravel the molecular mechanisms of drought responses in tomato, gene expression profiles of two drought-tolerant lines identified from a population of Solanum pennellii introgression lines, and the recurrent parent S. lycopersicum cv. M82, a drought-sensitive cultivar, were investigated under drought stress using tomato microarrays. Around 400 genes identified were responsive to drought stress only in the drought-tolerant lines. These changes in genes expression are most likely caused by the two inserted chromosome segments of S. pennellii, which possibly contain drought-tolerance quantitative trait loci (QTLs). Among these genes are a number of transcription factors and signalling proteins which could be global regulators involved in the tomato responses to drought stress. Genes involved in organism growth and development processes were also specifically regulated by drought stress, including those controlling cell wall structure, wax biosynthesis, and plant height. Moreover, key enzymes in the pathways of gluconeogenesis (fructose-bisphosphate aldolase), purine and pyrimidine nucleotide biosynthesis (adenylate kinase), tryptophan degradation (aldehyde oxidase), starch degradation (β-amylase), methionine biosynthesis (cystathionine β-lyase), and the removal of superoxide radicals (catalase) were also specifically affected by drought stress. These results indicated that tomato plants could adapt to water-deficit conditions through decreasing energy dissipation, increasing ATP energy provision, and reducing oxidative damage. The drought-responsive genes identified in this study could provide further information for understanding the mechanisms of drought tolerance in tomato.
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影响因子：
56.9
作者：
BOYER, JS
通讯作者：
BOYER, JS
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
11.6
作者：
Abe, H;Urao, T;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
14.9
作者：
Fei Z;Tang X;Alba R;Giovannoni J
通讯作者：
Giovannoni J
影响因子：
11.6
作者：
Aarts, MGM;Keijzer, CJ;Pereira, A
通讯作者：
Pereira, A