Degradation of pheromone and plant volatile components by a same odorant-degrading enzyme in the cotton leafworm, Spodoptera littoralis.
Degradation of pheromone and plant volatile components by a same odorant-degrading enzyme in the cotton leafworm, Spodoptera littoralis.
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DOI：
10.1371/journal.pone.0029147
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Maïbèche-Coisne M
中科院分区：
文献类型：
--
作者：
Durand N;Carot-Sans G;Bozzolan F;Rosell G;Siaussat D;Debernard S;Chertemps T;Maïbèche-Coisne M
气味降解酶（ODEs）被认为参与昆虫嗅觉感受器内的信号失活步骤，通过快速去除嗅觉受体附近的气味分子。只有三个ODE已被确定在分子水平上和功能上的特点：两个专门在信息素化合物的降解和最后一个被证明是降解植物气味。以前的工作表明，棉叶虫Spodoptera littoralis，一个世界性的农业作物害虫的触角，表达了许多候选ODE。我们专注于酯酶过表达的男性触角，即SlCXE 7。我们研究了它的表达模式，并测试了它对三种气味物质，即两种雌性信息素组分和一种寄主植物散发的绿色叶挥发物的催化特性。 SlCXE7的表达是伴随着在发展过程中与男性对气味的反应，并在成人暗期与男性最活跃的性行为的时期。此外，SlCXE 7的转录可以通过雄性暴露于主要信息素组分来诱导，这表明信息素降解酶的作用。有趣的是，重组SlCXE 7能够有效地水解信息素化合物，但也水解植物挥发物，对信息素的亲和力高于对植物化合物的亲和力。在男性触角，SlCXE 7的表达与长和短的感受器，调谐到性信息素或植物气味，分别。因此，我们的研究结果表明，同一个ODE可以有一个双重的功能依赖于它的感觉定位。在对信息素敏感的感受器中，SlCXE 7可能在信息素信号终止和气味背景噪声的降低中发挥作用，而在短感受器中，它可能参与植物气味失活。
Odorant-Degrading Enzymes (ODEs) are supposed to be involved in the signal inactivation step within the olfactory sensilla of insects by quickly removing odorant molecules from the vicinity of the olfactory receptors. Only three ODEs have been both identified at the molecular level and functionally characterized: two were specialized in the degradation of pheromone compounds and the last one was shown to degrade a plant odorant. Previous work has shown that the antennae of the cotton leafworm Spodoptera littoralis, a worldwide pest of agricultural crops, express numerous candidate ODEs. We focused on an esterase overexpressed in males antennae, namely SlCXE7. We studied its expression patterns and tested its catalytic properties towards three odorants, i.e. the two female sex pheromone components and a green leaf volatile emitted by host plants. SlCXE7 expression was concomitant during development with male responsiveness to odorants and during adult scotophase with the period of male most active sexual behaviour. Furthermore, SlCXE7 transcription could be induced by male exposure to the main pheromone component, suggesting a role of Pheromone-Degrading Enzyme. Interestingly, recombinant SlCXE7 was able to efficiently hydrolyze the pheromone compounds but also the plant volatile, with a higher affinity for the pheromone than for the plant compound. In male antennae, SlCXE7 expression was associated with both long and short sensilla, tuned to sex pheromones or plant odours, respectively. Our results thus suggested that a same ODE could have a dual function depending of it sensillar localisation. Within the pheromone-sensitive sensilla, SlCXE7 may play a role in pheromone signal termination and in reduction of odorant background noise, whereas it could be involved in plant odorant inactivation within the short sensilla.
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影响因子：
4.3
作者：
Gu Y;Lucas P;Rospars JP
通讯作者：
Rospars JP
DOI：
10.1073/pnas.0505340102
发表时间：
2005-09-27
影响因子：
11.1
作者：
Ishida, Y;Leal, WS
通讯作者：
Leal, WS
DOI：
10.1016/0020-1790(87)90093-x
发表时间：
1987-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
KLEIN, U
通讯作者：
KLEIN, U
DOI：
10.1073/pnas.0802610105
发表时间：
2008-07-01
影响因子：
11.1
作者：
Ishida, Yuko;Leal, Walter S.
通讯作者：
Leal, Walter S.
影响因子：
1.5
作者：
Jönsson, M;Anderson, P
通讯作者：
Anderson, P